单 位：
甘肃农业大学草业学院草业生态系统教育部重点实验室
关键词：
促生菌(PGPR);接种剂;箭筈豌豆;生长;根系
摘 要：
通过测定分离自箭筈豌豆和玉米根际4株细菌的固氮酶活性、溶磷量及分泌生长素能力,将其制成植物根际接种剂,并结合半固体培养试验测定接种剂对箭筈豌豆生长的影响。结果表明,菌株J3-1、J1-15和Y16具备溶磷和分泌生长素能力,J1-15的溶磷能力最强,为548.9mg/L,Y16分泌生长素能力最好,达17.8μg/mL,且菌株Y16具较强固氮能力,J3固氮酶活性为366.51C2H4nmol/(mL·h)。与对照组相比,单一菌株制备的接种剂处理(Y16)可使箭筈豌豆地上生物量、地下生物量分别显著增加104.5%和254.1%(P<0.05),复合接种剂处理F(J3-1+J1-15+Y16+J3)使箭筈豌豆地上和地下生物量分别增加76.1%和192.3%。综合各指标,复合接种剂处理效果明显优于单一接种剂,处理F(J3-1+J1-15+Y16+J3)可使箭筈豌豆株高、根长、根表面积、根体积、根系活力,分别较对照增加29.4%,70.0%,174.0%,194.6%,38.3%。这主要是由于菌种间的互作效应造成的。
译 名：
Assessment of rhizobacteria strains for Vicia sativa
作 者：
MA Wen-bin;YAO Tuo;WANG Guo-ji;ZHANG Yu-xia;RONG Liang-yan;Pratacultural College,Gansu Agricultural University,Agronomy College,Gansu Agricultrual University;
关键词：
plant growth promoting rhizobacteria(PGPR);;inoculum;;Vicia sativa;;growth;;root
摘 要：
To investigate the effect of rhizobacteria inoculants on the growth of Vicia sativa,four rhizobacteria strains(J3-1,J1-15,Y16 and J3)were assessed for potentially useful characteristics with the potential to enhance the growth and yield of V.sativa;high nitrogenase activity,high phosphate solubilisation and ability to secrete 3-indoleacetic acid(IAA)into the rhizosphere.Strains J3-1,J1-15 and Y16had the ability to solubilize phosphate and secrete IAA.J1-15,Y16,J3-1had the strongest phosphate solubilizing capacity(548.9mg/L),IAA secreting ability(17.8μg/mL),and nitrogenase activity(366.51C2H4nmol/mL·h),respectively.Y16 increased the aboveground biomass and underground biomass of V.sativa by 104.5%and 254.1%,respectively,compared to the non-inoculated control.An inoculant mixture of all four strains increased the aboveground biomass and underground biomass of V.sativa by 76.1%and 192.3%,respectively,relative to the control.This resulted from an increase in plant height,root length,root surface area,root volume and root activity of29.4%,70.0%,174.0%,194.6% and 38.3%,respectively.The results suggest that inoculants including mixtures of rhizobacteria strains may have significant benefits for V.sativa cultivation.
