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当前位置: 首页 > 文章 > 黄酮化合物对金黄葡萄球菌β-内酰胺酶活性影响 东北农业大学学报 2013,44 (3) 119-122
Position: Home > Articles > Effect of the flavonoids on the activity of β-lactamase from Staphylococcus aureus Journal of Northeast Agricultural University 2013,44 (3) 119-122

黄酮化合物对金黄葡萄球菌β-内酰胺酶活性影响

作  者：
刘立新;高月林;王朝兴;张羽男
单  位：
黑龙江省教育厅生物药制剂重点实验室;黑龙江农业职业技术学院
关键词：
黄酮类化合物;金黄葡萄球菌;β-内酰胺酶;活性
摘  要：
试验旨在筛选黄酮类化合物,研究其对金黄葡萄球菌β-内酰胺酶活性影响。利用试管二倍稀释法测定黄酮类化合物对耐药金黄葡萄球菌MIC值;利用K-B纸片法筛选黄酮类化合物;利用反复冻融法提取β-内酰胺酶,并用酶标仪测定β-内酰胺酶活性。结果表明,芦丁、姜黄素和槲皮素分别作用于耐药金黄葡萄球菌后,其β-内酰胺酶活性分别为2.36,2.78和3.05 U.mg-1,与阴性对照组相比差异极显著(P<0.01)。提示黄酮类化合物能降低金黄葡萄球菌β-内酰胺酶活性,能逆转金黄葡萄球菌耐药性。
译  名：
Effect of the flavonoids on the activity of β-lactamase from Staphylococcus aureus
作  者：
Liu Lixin1,Gao Yuelin2,Wang Chaoxing1,Zhang Yunan1(1.The Key Laboratory for Biological Drug of the Education Department Helongjiang,College of Pharmacy,Jiamusi University,Jiamusi Heilongjiang 154007,China;2.Heilongjiang Agricultural vocational and technical college,Jiamusi Heilongjiang 154007,China)
关键词：
Flavonoids;Staphylococcus aureus;β-lactamase;Activities
摘  要：
Effects of the flavonoids on the activity of Staphylococcus aureus β-lactamase were studied by screening flavonoids.The minimal inhibitory concentration(MIC) of teicoplanin was determined by tube dilution method,flavonoids were screened by means of K-B paper methods.β-lactamase was prepared by repetitive freeze thaw method,enzyme-labelling measuring instrument was used to measure the activity of Staphylococcus aureus β-lactamase.Rutin,curcumin and quercetin were acted on Staphylococcus aureus respectively,which showed that the activities of β-lactamase were 2.36,2.78 and 3.05 U.mg-1.Compared with negative control group,differences were remarkable(P<0.01).The results show that the flavonoids could decrease the activity of β-lactamase and reverse the drug resistance of staphylococcus aureus.

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