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当前位置: 首页 > 文章 > 液相色谱串联质谱法检测食品中的黄曲霉毒素 食品科学 2010,31 (24) 385-388
Position: Home > Articles > Determination of Aflatoxins in Food by Liquid Chromatography-Tandem Mass Spectrometry FOOD SCIENCE 2010,31 (24) 385-388

液相色谱串联质谱法检测食品中的黄曲霉毒素

作  者：
郑燕;王远兴;李瑾瑾
单  位：
南昌大学食品科学与技术国家重点实验室
关键词：
液相色谱串联质谱;黄曲霉毒素;食品
摘  要：
为提高黄曲霉毒素的检测灵敏度,建立快速液相色谱串联质谱(LC-MS/MS)法对食品中的4种黄曲霉毒素B1、B2、G1、G2进行定性定量分析。样品粉碎后用体积比为84:16的乙腈-水混合液提取,过滤后通过真菌毒素净化柱进样,采用C18柱分离,0.1%甲酸溶液和甲醇做流动相,以60:40比例等度洗脱,质谱在多反应监测(MRM)的正离子模式下进行分析。4种组分在5min内完全分离,而且此方法线性关系良好,黄曲霉毒素B1、B2、G1、G2的检出限分别是0.012、0.009、0.013、0.007μg/kg,平均加标回收率在80%～95%之间,相对标准偏差小于5%。该方法快速灵敏、准确可靠,其检出限可满足欧盟地区严格的黄曲霉毒素限量标准。
译  名：
Determination of Aflatoxins in Food by Liquid Chromatography-Tandem Mass Spectrometry
作  者：
ZHENG Yan,WANG Yuan-xing,LI Jin-jin (State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China)
关键词：
liquid chromatography-tandem mass spectrometry;aflatoxins;food
摘  要：
In order to improve detection sensitivity of aflatoxions, a liquid chromatography-tandem mass spectrometry method was established for the simultaneously qualitative and quantitative analysis of aflatoxins B1, B2, G1 and G2. Samples were sequentially extracted by acetonitrile-water at a ratio of 84:16 (V/V), cleaned up with multifunctional cleaning column. After injection, the chromatographic separation was achieved on a C18 column using a mobile phase composed of a mixture of 0.1% formic acid and methanol through gradient elution. The analysis was realized by multiple reaction-monitoring modes. Under above conditions, aflatoxins B1, B2, G1 and G2 could be completely separated in 5 min with an excellent linear relationship. The determination limits for 4 kinds of aflatoxins were 0.012, 0.009, 0.013μg/kg and 0.007μg/kg, respectively. The recovery rate of spiked samples was in the range of 80%-95% with a relative standard deviation less than 5%. This method can provide a rapid, sensitive, accurate and reproducible detection for aflatoxins and its detection limit is sensitive enough to meet the European regulation for aflatoxins.

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