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当前位置: 首页 > 文章 > 供体细胞系基因座位特异DNA甲基化多态性及其与猪克隆效率的关联分析 广东农业科学 2014,41 (12) 165-170
Position: Home > Articles > Study on locus-specific DNA methylation polymorphisms among donor cell lines and their associations with porcine somatic cell cloning efficiency Guangdong Agricultural Sciences 2014,41 (12) 165-170

供体细胞系基因座位特异DNA甲基化多态性及其与猪克隆效率的关联分析

作  者：
许卫华;吴珍芳;余波;贺晓燕;石俊松;周荣;刘德武;李紫聪
单  位：
华南农业大学动物科学学院/广东省农业动物基因组学与分子育种重点实验室;广东省温氏集团研究院
关键词：
猪;供体细胞;DNA甲基化;多态性;克隆效率
摘  要：
利用甲基化限制性酶切试验(Combined bisulfite restriction analysis,COBRA)和亚硫酸盐转换PCR(Bisulfite sequencing PCR,BSP)后测序的方法,筛选猪体细胞克隆优势供体细胞提供基因座位特异的DNA甲基化遗传标记。比较8个发育重要基因的差异甲基化区域(Differentially methylated regions,DMRs)在不同克隆供体细胞系中的DNA甲基化多态性,并与猪克隆成绩进行关联分析。结果表明:除了XIST-DMR2和H19-DMR3 DNA甲基化变异幅度较小(低于10%)外,其他基因的相关座位均存在一定的COBRA多态性,其中,LINE1-DMR1、POU5F1-DMR1和NANOG-DMR1等3个差异甲基化化区域在克隆效率最高细胞系中处均处于最低程度的DNA甲基化状态,因此,可作为筛选猪体细胞克隆优势供体细胞系的潜在的DNA甲基化遗传标记。
译  名：
Study on locus-specific DNA methylation polymorphisms among donor cell lines and their associations with porcine somatic cell cloning efficiency
作  者：
XU Wei-hua;WU Zhen-fang;YU Bo;HE Xiao-yan;SHI Jun-song;ZHOU Rong;LIU De-wu;LI Zi-cong;Colleg of Animal Science,South China Agricultural University / Guangdong Provincial Key Lab of Agro-animal Genomics and Molecular Breeding;College of Life Sciences,Longyan University;Institute of Wen's Group;
关键词：
porcine;;donor cell;;DNA methylation;;polymorphisms;;cloning efficiency
摘  要：
Bisulfite restriction analysis(COBRA) combined with bisulfite sequencing(BS) were firstly carried out to screen locus-specific DNA methylation markers in donor cell lines for improving porcine somatic cell cloning efficiency.DNA methylation polymorphisms of eight differentially methylated regions(DRMs) among six donor cell lines and their potential associations with porcine cloning achievements were analyzed. Result indicated that all DMRs presented relatively high degree of DNA methylation polymorphisms, except for XIST-DMR2 and H19-DMR3 with low extent of DNA methylation variations below 10%. Moreover, three DMRs including LINE1-DMR1, POU5F1-DMR1, and NANOG-DMR1presented the most DNA hypomethylated status in the most efficient cell line, and thus could be considered as potential DNA methylation genetic markers for selecting optimum donor cell lines for porcine somatic cell cloning.

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