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Chinese Journal of Preventive Veterinary Medicine
2012,34
(12)
967-971
家兔胚胎胃肠道细菌分离及16S rRNA基因序列分析
作 者：
郭海勇;单晓枫;吴静;孙文怡;于超;王好;钱爱东
单 位：
吉林师范大学生命科学学院;吉林农业大学动物科学技术学院
关键词：
家兔胚胎;胃肠道;细菌分离;16SrRNA基因序列
摘 要：
为研究家兔胚胎胃肠道细菌菌群的种类,对常规饲养、正常妊娠的家兔胚胎后期胃肠道细菌进行分离,基于分离细菌的生理生化特性、16S rRNA基因序列的同源性比较及系统发育分析,初步确定家兔胚胎后期胃肠道中存在的微生物菌群有微球菌属(Micrococcus)、葡萄球菌属(Staphylococcus)、棒杆菌属(Corynebacterium)以及蜡样芽孢杆菌(Bacillus cereus)等细菌,其序列同源性均为97%～99%。分离细菌均为G+细菌,家兔胚胎胃肠道中细菌的平均数量达到0.8×102cfu/g～1.2×102cfu/g,本研究为哺乳动物胚胎时期存在于胃肠道中细菌的深入研究奠定了基础。
译 名：
Isolation and 16S rRNA gene sequence analysis of gastrointestinal bacteria in the rabbit embryos
作 者：
GUO Hai-yong1,SHAN Xiao-feng2,WU Jing2,SUN Wen-yi1,YU Chao2,WANG Hao2,Qian Ai-dong2*(1.College of Life Sciences,Jilin Normal University,Siping 136000,China; 2.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)
关键词：
rabbit embryos;gastrointestinal tract;bacterial isolation;16S rRNA gene sequence
摘 要：
In order to study the variety of gastrointestinal bacteria in the rabbit embryos,the bacteria of gastrointestinal tract in the later embryonic period were isolated from the conventional feeding and normal pregnant rabbits.Based on the bacterial physiological and biochemical properties,homology and phylogenetic analysis of 16S rRNA gene sequence,four isolates were identified as the genera Micrococcus,Staphylococcus,Corynebacterium and Bacillus cereus,all of which have 97% to 99% similarity.The bacteria were the gram-positive bacterium and the average number of bacteria was 0.8×102 cfu/g to 1.2×102 cfu/g in gastrointestinal tract of the rabbit embryo in the later embryonic period.This study would lay a foundation for further studies on bacteria of gastrointestinal tract from the mammalian embryo stage.
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