Position: Home > Articles > Investigation of fermentation conditions for microbial oil production by Lipomyces kononenkoae
Chinese Journal of Oil Crop Sciences
2012,34
(1)
82-86+95
橘林油脂酵母发酵条件的初步研究
作 者：
张东辉;梁焯;万霞;龚阳敏;江木兰
单 位：
中国农业科学院油料作物研究所
关键词：
橘林油脂酵母;单因素实验;发酵条件;微生物油脂
摘 要：
以橘林油脂酵母(Lipomyces kononenkoae CICC1714)为实验材料,通过设计单因素实验,确定了摇瓶发酵培养的最佳产油条件为:氮源为硫酸铵,碳源为葡萄糖,培养温度28℃,接种量10%,初始pH值为7.0,最适碳氮比为99。优化后批次发酵的生物量为7.01g/L,油脂含量15.91%,油脂产量1.12g/L。经半连续发酵后最终的生物量为24.13g/L,油脂产量为3.06g/L。
译 名：
Investigation of fermentation conditions for microbial oil production by Lipomyces kononenkoae
作 者：
ZHANG Dong-hui1,LIANG Zhuo1,WAN Xia1,2,GONG Yang-min1,2,JIANG Mu-lan1,2(1.Oil Crops Research Institute of Chinese Academy of Agriculture Sciences,Wuhan 430062,China; 2.Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,China)
关键词：
Lipomyces kononenkoae;Single-factor experiment;Fermentation condition;Microbial oil
摘 要：
The oil-producing conditions of Lipomyces kononenkoae were optimized on flask fermentation level by the single-factor experiment.The highest lipid yield was obtained with ammonium sulfate and glucose as nitrogen source and carbon source,respectively.The optimum culture conditions for temperature,inoculation,initial pH value and C/N ratio were 28℃,10%,7.0 and 99,respectively.Batch culture result showed that the biomass,lipid content and lipid yield of Lipomyces kononenkoae could reach 7.01g/L,15.91%,1.12g/L respectively after optimization.The results of flask fed-batch cultures indicated the dry biomass and lipid yield of Lipomyces kononenkoae could further reach 24.13g/L and 3.06g/L respectively.
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