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当前位置: 首页 > 文章 > 菲和芘胁迫下AMF和PGPR对高羊茅生理生态的响应 草业学报 2019 (8) 84-94
Position: Home > Articles > 菲和芘胁迫下AMF和PGPR对高羊茅生理生态的响应 Acta Prataculturae Sinica 2019 (8) 84-94

菲和芘胁迫下AMF和PGPR对高羊茅生理生态的响应

作  者：
李文彬;宁楚涵;李伟;李峰;郭绍霞
单  位：
青岛农业大学菌根生物技术研究所
关键词：
丛枝菌根真菌;根围促生细菌;菲和芘;光合;叶绿素;生理
摘  要：
旨在探究菲和芘胁迫下丛枝菌根真菌(AMF)与根围促生细菌(PGPR)对高羊茅生理生态的响应.以菲和芘1∶1混合处理浓度各0、50、100和150 mg·kg-1下对高羊茅接种AMF变形球囊霉(Gv)、PGPR荧光假单胞菌(Ps2-6)、Gv+Ps2-6和不接种对照共16个处理.结果表明,AMF、PGPR或AMF+PGPR处理均显著增加高羊茅生物量和菌根侵染率,增强植物光合作用,提高叶绿素含量,显著提高植物体内生理活性.在土壤中菲和芘100 mg·kg-1水平下,与对照相比,双接种Gv+Ps2-6处理的高羊茅叶片叶绿素a、b和总叶绿素含量分别提高57.7%、41.7%和51.8%;净光合速率(Pn)、蒸腾速率(Tr)、胞间CO2浓度(Ci)和气孔导度(Gs)分别增加70.6%、100.0%、4.5%和78.6%;最大光化学效率(Fv/Fm)和PSⅡ潜在活性(Fv/Fo)分别提高2.2%和8.8%;超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性、可溶性糖和脯氨酸含量分别是对照的1.6、1.5、2.3和2.7倍,丙二醛含量比对照下降46.0%;高羊茅株高和地上鲜重分别比对照增加63.0%和69.6%;接种PGPR处理能够增加AMF侵染率以及菌根依赖性.供试条件下,双接种Gv+Ps2-6处理增加高羊茅叶绿素含量和抗氧化能力,增强光合作用能力,降低膜脂过氧化水平,促进植物生长的作用最为显著.
作  者：
LI Wen-bin;NING Chu-han;LI Wei;LI Feng;GUO Shao-xia;Institute of Mycorrhizal Biotechnology, Qingdao Agricultural University;College of Landscape Architecture and Forestry, Qingdao Agricultural University;
关键词：
arbuscular mycorrhizal fungi;;plant growth-promoting rhizobacteria;;phenanthrene and pyrene;;photosynthesis;;chlorophyll;;physiology
摘  要：
The purpose of the present study was to evaluate mechanisms(and their efficiency) for degradation of residues of phenanthrene and pyrene by arbuscular mycorrhizal fungi(AMF) and plant growth-promoting rhizobacteria(PGPR). A total of 16 treatments were designed involving Festuca elata, with or without inoculation with AMF Glomus versiforme(Gv), PGPR Pseudomonas fluorescens(Ps2-6), or Gv+Ps2-6, and grown soil with 0(Control), 50, 100 or 150 mg·kg~(-1) added polycyclic aromatic hydrocarbons(PAHs)(phenanthrene∶pyrene=1∶1 in weight). The results showed that inoculation with AMF, PGPR or AMF+PGPR significantly increased the biomass and mycorrhizal infection rate of tall fescue, enhanced photosynthesis and chlorophyll content of plants, and significantly increased the physiological activity of plants. At 100 mg·kg~(-1) phenanthrene and pyrene, the chlorophyll a, b and total chlorophyll content in the leaves of tall fescue were increased by 57.7%, 41.7% and 51.8%, respectively, compared with the Control, and the net photosynthetic rate(P_n), transpiration rate(T_r), intercellular CO_2 concentration(C_i) and gas pore conductivity(G_s) were increased by 70.6%, 100.0%, 4.5% and 78.6%, respectively; the maximum photochemical efficiency(F_v/F_m) and PSⅡ potential activity(F_v/F_o) were increased by 2.2% and 8.8%, respectively. The activities of superoxide dismutase(SOD), catalase(CAT), soluble sugar and proline were, respectively, 1.6, 1.5, 2.3 and 2.7 times those of the Control; the malondialdehyde content was decreased by 46.0%, and the height and fresh weight were increased by 63.0% and 69.6%. Inoculation with PGPR increased AMF infection rate and mycorrhizal dependency. Under the test conditions, double inoculation with a Gv+Ps2-6 treatment increased the content of photosynthetic pigments and antioxidant capacity of Festuca elata, enhanced photosynthetic capacity, reduced the level of membrane lipid peroxidation, and significantly promoted plant growth.

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