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Position: Home > Articles > Pretreatment of Fermented Mash Containing Poly-γ-glutamic acid(γ-PGA) FOOD SCIENCE 2013,34 (21) 168-171

γ-聚谷氨酸发酵液预处理的研究

作  者:
梁金钟;张露露;王风青
单  位:
黑龙江省高等学校食品科学与工程重点实验室,哈尔滨商业大学食品工程学院;黑龙江省高等学校食品科学与工程重点实验室
关键词:
γ-聚谷氨酸(γ-PGA);硅藻土;除菌体
摘  要:
为了提高γ-聚谷氨酸(γ-PGA)的质量,基于γ-PGA发酵液的物化特性,采用单因素试验逐个确定影响硅藻土除杂质的因子的最佳条件,最后在单因素试验的基础上用硅藻土层模拟板框模式过滤除菌体。以除菌率、杂蛋白质量浓度、γ-PGA质量浓度为指标,研究硅藻土的用量。结果表明:发酵液加热至50℃,4倍稀释,以1.6cm厚(约25g)的硅藻土层作为滤饼层,连续过滤4次后,能除去130mL原发酵液中几乎全部的菌体,发酵液中蛋白质质量浓度为0.32mg/mL,γ-PGA的损失率为34.8%。
译  名:
Pretreatment of Fermented Mash Containing Poly-γ-glutamic acid(γ-PGA)
作  者:
LIANG Jin-zhong;ZHANG Lu-lu;WANG Feng-qing;Key Laboratory of Food Science and Engineering,College of Food Engineering,Harbin University of Commerce;
关键词:
poly-γ-glutamic acid(γ-PGA);;diatomite;;bacterial cells
摘  要:
In order to improve the quality of poly-γ-glutamic acid(γ-PGA),single factor experiments were used to determine the best conditions for removing bacterial cells through diatomite,based on the physical and chemical properties of fermented mash containing γ-PGA.The amount of diatomite was determined based on bacterial removal,protein concentration,γ-PGA concentration.The results showed that when the fermented mash was heated at 50 ℃,diluted 4-fold and then f iltered through 1.6 cm thickness(approximately 25 g) diatomite four times,the bacterial cells were almost completely removed from 130 mL of γ-PGA fermented mash.The protein concentration was 0.32 mg/mL,and the loss of γ-PGA was 34.8%.

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