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当前位置: 首页 > 文章 > 不同地区萧氏松茎象遗传多样性的RAPD分析 中南林业科技大学学报 2012,32 (5) 149-154
Position: Home > Articles > RAPD analysis on genetic diversity of Hylobitelus xiaoi Zhang from different geographical population in China Journal of Central South University of Forestry & Technology 2012,32 (5) 149-154

不同地区萧氏松茎象遗传多样性的RAPD分析

作  者：
李国宏;王伟;赵同海
单  位：
中国林业科学研究院森林生态环境与保护研究所
关键词：
萧氏松茎象;地理种群;遗传差异;随机扩增多态性DNA
摘  要：
以萧氏松茎象7个地理种群的基因组DNA为材料,对其进行随机扩增多态性DNA(RAPD)分析,从80个引物中筛选出39个稳定性好、多态性高的引物。7个地理种群共扩增出489条扩增条带,每条引物平均可以得到12.5条扩增条带,其长度从250～2 000 bp不等,其中443条(90.6%)扩增条带为多态性条带,平均每条引物可得到11.4条多态性条带。通过公式计算多态性位点、遗传距离、及Nei's遗传距离,结果表明:萧氏松茎7个地理种群之间的遗传距离指数的变异范围为0.403 7～0.484 4,遗传相似性指数为0.515 6～0.596 3,平均遗传距离指数为0.444 1;其Shannon's信息指数为0.588 6～0.625 7,Nei's遗传距离为0.009 0～0.030 2;表明萧氏松茎象种群间存在一定的遗传多样性。通过UPGMA聚类分析,将7个萧氏松茎象地理种群划分为4个聚类簇。分析表明,萧氏松茎象7个地理种群存在丰富的遗传多样性,其遗传分化与地理位置有一定的相关性。
译  名：
RAPD analysis on genetic diversity of Hylobitelus xiaoi Zhang from different geographical population in China
作  者：
LI Guo-hong,WANG Wei,ZHAO Tong-hai(Research Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry,Beijing 100091,China)
关键词：
Hylobitelus xiaoi Zhang;geographic population;genetic diversity;RAPD
摘  要：
The genetic variation of seven geographic populations of H.xiaoi were detected by RAPD.A total of 489 bands(from 250 bp to 2 000 bp) were amplified by using 39 primers screened from 80 random primers of which 443 bands(90.6%) were polymorphic,thus the average number of polymorphic bands was 11.4 per primer.The data analysis show that the genetic comparability of the seven geographic populations varied from 0.515 6 to 0.596 3 and the range of genetic distance was from 0.403 7 to 0.484 4,with the average 0.444 1.The Shannon's information index was from 0.588 6 to 0.625 7 and the Nei's genetic distance was from 0.009 0 to 0.030 2.The seven geographical populations were classified into four cluster groups by UPGMA method.The studying results revealed that the genetic diversity and homology existed between geographic populations of H.xiaoi.

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