当前位置: 首页 > 文章 > 核酸等温扩增技术在微生物快速检测中的研究进展 生物技术通报 2017 (7) 49-61
Position: Home > Articles > Research Progress on the Isothermal Nucleic Acid Amplification Techniques in Rapid Detection of Microorganisms Biotechnology Bulletin 2017 (7) 49-61

核酸等温扩增技术在微生物快速检测中的研究进展

作  者:
王大洲;郭天笑;郑实;商颖;许文涛
单  位:
昆明理工大学食品安全研究院;中国农业大学食品科学与营养工程学院
关键词:
等温扩增;微生物;核酸;快速检测
摘  要:
等温扩增技术在近20多年,得到了快速发展,依靠其等温反应的优势正逐渐取代传统的体外扩增技术聚合酶链式反应(Ploymerase chain reaction,PCR),其已经在临床医学、检验医学、分子生物学、基因组学、食品安全等不同领域中的发挥着重要作用,特别是在致病菌、病毒等微生物的检测中。将选取10余种关注度较高的等温扩增技术,主要针对其反应原理、优缺点以及发展应用等方面进行简要概述和对比,最后展望了等温扩增技术未来的发展趋势,以期对相关研究领域的发展起到积极的促进作用。
译  名:
Research Progress on the Isothermal Nucleic Acid Amplification Techniques in Rapid Detection of Microorganisms
作  者:
WANG Da-zhou;GUO Tian-xiao;ZHENG Shi;SHANG Ying;XU Wen-tao;Institute of Food Safety,Kunming University of Science and Technology;College of Food Science and Nutritional Engineering,China Agricultural University;
关键词:
isothermal amplification;;microorganisms;;nucleic acid;;rapid detection?
摘  要:
In recent 20 years,isothermal amplification technology has been rapidly developed. Relying on the advantage of theisothermal reaction,it is gradually replacing the traditional in vitro PCR(polymerase chain reaction)amplification,and plays an importantrole in different fields such as clinical medicine,laboratory medicine,molecular biology,genomics,food safety,and especially in thedetection of pathogenic bacteria,viruses and other microorganisms. This article reviews and compares more than 10 kinds of high-profileisothermal amplification technologies,mainly focusing on the reaction principles,advantages,disadvantages as well as the development andapplication,and finally prospects the trends of isothermal amplification techniques,in order to play a positive role in the development of therelated research fields.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊