中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 胡黄连与牛蒡子有效成分对猪磷酸二酯酶7A2活性的影响 北京农学院学报 2014,29 (4) 69-72
Position: Home > Articles > Effects of fructus arctii and picrorhiza effictive component on porcine phosphodiesterase 7A2 activity Journal of Beijing University of Agriculture 2014,29 (4) 69-72

胡黄连与牛蒡子有效成分对猪磷酸二酯酶7A2活性的影响

作  者：
付源;李佳;孙胜永;姜代勋;陈武
单  位：
北京农学院动物科学技术学院/兽医学(中医药)北京市重点实验室
关键词：
猪;磷酸二酯酶7A2;胡黄连;牛蒡子
摘  要：
从牛蒡子、胡黄连中筛选对猪磷酸二酯酶(PDE)7A2具有抑制作用的中药单体成分,为PDE亚型抑制剂研究积累资料。使用高效液相色谱法(HPLC)测定绿原酸、牛蒡子苷、β-谷甾醇和胡黄连苷-Ⅱ对体外原核表达的猪PDE7A2的抑制作用。结果表明:在反应质量浓度为1,5,10,50,100μmol/L时,绿原酸对猪PDE7A2的活性抑制率分别为12.55%,46.29%,56.17%,68.52%,47.12%;牛蒡子苷抑制率分别为21.60%,41.35%,70.16%,88.27%,80.07%;β-谷甾醇抑制率分别为40.53%,54.52%,71.81%,96.50%,94.86%;胡黄连苷-Ⅱ抑制率分别为69.34%,94.03%,96.50%,103.09%,101.44%。绿原酸、牛蒡子苷、β-谷甾醇和胡黄连苷-Ⅱ对体外表达的猪PDE7A2均有抑制作用,其中胡黄连苷-Ⅱ对猪PDE7A2的抑制作用最强。
译  名：
Effects of fructus arctii and picrorhiza effictive component on porcine phosphodiesterase 7A2 activity
作  者：
FU Yuan;LI Jia;SUN Shengyong;JIANG Daixun;CHEN Wu;College of Animal Science and Technology,Beijing University of Agriculture/Beijing Key Laboratory of Traditional Chinese Veterinary Medicine,Beijing University of Agriculture;
关键词：
porcine;;phosphodiesterase 7A2;;fructus arctii;;picrorhiza
摘  要：
[Purpose]The aims of this paper were to screen the monomers of Chinese traditional herbs Fructus Arctii and Picrorhiza which could inhibit Porcine PDE7A2 activity.[Methods]The inhibiting effects of chlorogenic acid,arctiin which the effictive component of Picrorhiza andβsitosterol,picroside II which the effictive component of Fructus Arctii on in vitro prokaryotic expressed Porcine PDE7A2 were determined by High Performance Liquid Chromatography(HPLC).[Results]With the reaction concentrations of1μmol/L,5μmol/L,10μmol/L,50μmol/L and 100μmol/L,the activity inhibiting ratios of chlorogenic acidare 12.55%,46.29%,56.17%,68.52% and 47.12%respectively;those of arctiin are 21.60%,41.35%,70.16%,88.27%and 80.07%respectively;those ofβsitosterol are 40.53%,54.52%,71.81%,96.50%and 94.86%respectively,and those of picroside II are 69.34%,94.03%,96.50%,103.09%and101.44%respectively.[Conclusion]Chlorogenic acid,arctiin,βsitosterol and picroside II all had inhibiting effects on Porcine PDE7A2 in vitro prokaryotic expression,among which picroside II had strongest inhibiting function.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46