作 者：
王玉荣;杨成聪;葛东颖;尚雪娇;张振东;郭壮
单 位：
湖北文理学院食品科学技术学院鄂西北传统发酵食品研究所
关键词：
鲊广椒;引物;扩增区域;MiSeq测序;细菌多样性
摘 要：
在使用引物27F/338R、338F/806R和515F/907R分别对细菌16S rRNA基因的V_1～V_2区、V_3～V_4区和V_4～V_5区进行扩增的基础上,采用MiSeq高通量测序评价扩增区域对鲊广椒细菌多样性分析结果的影响。结果发现,扩增16S rRNA基因V_4～V_5区的非特异性序列所占比例显著偏低(P<0.05),而扩增V_1～V_2区样品细菌的Chao 1指数显著偏高(P<0.05)。虽然扩增16S rRNA基因的V_3～V_4区会导致Cyanobacteria(蓝细菌门)和Pediococcus(小球菌)相对含量显著偏高(P<0.05),但不同扩增区域扩增出的同一样品其微生物群落结构无显著差异(P>0.05)。在对鲊广椒细菌多样性进行解析时,建议选择引物515F/907R扩增16S rRNA基因的V_4～V_5区。
译 名：
In?uence of Different Ampli?ed Regions on Results of Bacterial Diversity in Zhaguangjiao,a Chinese Traditional Fermented Chili Product, by MiSeq Sequencing
作 者：
WANG Yurong;YANG Chengcong;GE Dongying;SHANG Xuejiao;ZHANG Zhendong;GUO Zhuang;Northwest Hubei Research Institute of Traditional Fermented Food, College of Food Science and Technology,Hubei University of Arts and Science;
单 位：
WANG Yurong%YANG Chengcong%GE Dongying%SHANG Xuejiao%ZHANG Zhendong%GUO Zhuang%Northwest Hubei Research Institute of Traditional Fermented Food, College of Food Science and Technology,Hubei University of Arts and Science
关键词：
Zhaguangjiao;;primer;;amplified regions;;MiSeq sequencing;;bacterial diversity
摘 要：
This study aimed to evaluate whether different amplified regions of 16 S rRNA gene influence the results of bacterial diversity in Zhaguagnjiao by MiSeq sequencing. The V_1–V_2, V_3–V_4 and V_4–V_5 regions were amplified using bacterial genomic DNA extracted from Zhaguangjiao samples as template with three different primer pairs, 27 F/338 R,338 F/806 R and 515 F/907 R. The results indicated that the percentage of non-specific amplification sequences were signi?cantly lower in the ampli?ed products of V_4–V_5 region(P < 0.05), and the Chao 1 index was signi?cantly higher in the ampli?ed products of V_1–V-2 regions(P < 0.05). Although the relative abundance of Cyanobacteria and Pediococcus were signi?cantly higher in the ampli?ed products of V_3–V_4 region(P < 0.05), there was no signi?cant difference in the microbial community structure of the same samples with different ampli?ed variable regions(P > 0.05). Thus, ampli?cation of the V_4–V_5 region using primer pair 515 F/907 R is recommended for analysis of the bacterial diversity of Zhaguangjiao.
