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当前位置: 首页 > 文章 > 利迪链霉菌E12发酵液及其粗提物的抑菌活性初探 农药学学报 2016,18 (2) 258-262
Position: Home > Articles > Study on the antagonistic activity of fermentation broth and its crude extract produced by Streptomyces lydicus E12 Chinese Journal of Pesticide Science 2016,18 (2) 258-262

利迪链霉菌E12发酵液及其粗提物的抑菌活性初探

作  者：
陈恳
单  位：
天津大学化工学院
关键词：
利迪链霉菌;抑菌活性;β-1,3-葡聚糖酶;聚丙烯酰胺凝胶
摘  要：
利迪链霉菌E12的发酵液对多种植物病原真菌具有较强的抑制活性。采用琼脂稀释法考察了淀粉和葡萄糖对利迪链霉菌发酵液抑菌活性的影响;采用大孔树脂吸附层析和浓缩沉淀提取发酵液中的抑菌活性物质;利用聚丙烯酰胺凝胶(SDS-PAGE)与含β-1,3-葡聚糖的琼脂糖凝胶直接反应测定粗提物的分子质量及β-1,3-葡聚糖酶活性并采用琼脂扩散法测定其抑菌活性。结果表明:当以淀粉作为惟一碳源时发酵液的抑菌率为89.2%,当以葡萄糖作为惟一碳源时发酵液无抑菌活性;在SDS-PAGE上仅出现1条大小为3 k Da左右的条带,且该条带具有抑菌活性,与其位置对应的琼脂糖凝胶上出现透明带。以上结果表明,利迪链霉菌E12可能产生一种具有β-1,3-葡聚糖酶活性的抑菌活性物质。
译  名：
Study on the antagonistic activity of fermentation broth and its crude extract produced by Streptomyces lydicus E12
作  者：
CHEN Ken;School of Chemical Engineering, Tianjin University;
关键词：
Streptomyces lydicus;;antimicrobial activity;;β-1,3-glucanase;;SDS-PAGE
摘  要：
Fermentation broth of Streptomyces lydicus E12 displayed strong antagonistic activity against some pathogenic fungi. Effect of starch and glucose on the antifungal activity of fermentation broth was first explored by the agar diffusion method. Then antifungal substance was extracted from the fermentation broth by macroporous resin column chromatagrophy and gravity thickening. The molecular weight and β-1,3-glucanase activity of the antifungal crude extract was detected through the reaction between the polyacrylamide gel and a thin agar gel containing β-1,3-glucan. Results showed that the inhibition ratio of the fermentation broth dropped from 89.2% to zero when starch was replaced by glucose in the media as the solar carbon source. There was only one band on the SDS-PAGE gel with a molecular weight of about 3 k Da which showed antifungal activity, and at the corresponding position on the agar gel a clear band appeared. Take together, Streptomyces lydicus E12 can produce an antifungal substance with β-1,3-glucanase activity.

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