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Position: Home > Articles > The Pathogen Detection of Contagious caprine pleuropneumonia Hubei Agricultural Sciences 2011,50 (2) 340-341

山羊传染性胸膜肺炎病原的检测

作  者:
赵萍;郭晗;储岳峰;贺英;高鹏程;张念章;逯忠新
单  位:
中国农业科学院兰州兽医研究所/家畜疫病病原生物学国家重点实验室/农业部草食动物疫病重点开放实验室/农业部畜禽病毒学重点开放实验室
关键词:
山羊传染性胸膜肺炎;山羊支原体山羊肺炎亚种;PCR
摘  要:
湖北某地羊场发生严重呼吸道疾病,经初步诊断为疑似山羊传染性胸膜肺炎(CCPP)。将患病山羊肺组织提取基因组DNA,以此为模板根据OIE推荐的PCR-REA方法和Mccp-specificPCR方法进行检测,结果这两种方法都得到了预期的片段。说明送检山羊肺组织中存在山羊支原体山羊肺炎亚种(Mccp),也证实了国内Mccp的流行。
译  名:
The Pathogen Detection of Contagious caprine pleuropneumonia
作  者:
ZHAO Ping,GUO Han,CHU Yue-feng,HE Ying,GAO Peng-cheng,ZHANG Nian-zhang,LU Zhong-xin(Lanzhou Veterinary Research Institue(LVRI),Chinese Academy of Agricultural Sciences/State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture/Key Laboratory of Animal Virology of Ministry of Agriculture,Lanzhou 730046,China)
关键词:
contagious caprine pleuropneumonia;mycoplasma capricolum subsp.capripneumoniae;PCR
摘  要:
Goats of one sheep farm in Hubei had respiratory infectious disease.These goats had Contagious caprine pleuropneumonia by preliminary clinical diagnosis.We extracted the sick goats lung tissue's genomic DNA and did PCR-REA and Mccp-specific PCR recommended by OIE.The prospective fragments were received through both methods.It showed that Mccp was extisted in the sick goats lung tissue and was epidemic in China.

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