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Position: Home > Articles > Refluxing Extraction of Bergenin from Astilbe chinensis Journal of Northwest Forestry University 2012,27 (4) 186-189

落新妇中岩白菜素的回流提取工艺研究

作  者:
王瑾;马延;王占红;刘燕;汪晶;张跃进
单  位:
陕西省食品药品监督管理局;西北农林科技大学生命科学学院
关键词:
落新妇;岩白菜素;回流提取;HPLC法
摘  要:
研究回流法提取落新妇中岩白菜素的最佳工艺条件。以岩白菜素得率为指标,在单因素试验的基础上,通过正交试验,考察不同溶剂(A)、提取时间(B)、料液比(C)、提取次数(D)4个因素对落新妇中岩白菜素得率的影响。结果表明:通过建立落新妇中岩白菜素含量的HPLC测定方法,回流提取最佳工艺条件为:A2B3C1D2,即45%乙醇作为提取溶剂,提取时间为2.5h,料液比为1︰20,提取次数为2次。在此最佳参数组合下,岩白菜素得率为2.41%。
译  名:
Refluxing Extraction of Bergenin from Astilbe chinensis
作  者:
WANG Jin1,MA Yan1,WANG Zhan-hong1,LIU Yan2,WANG Jing2,ZHANG Yue-jin1(1.College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China; 2.Shaanxi Food and Drug Administration,Xi′an,Shaanxi 710065,China)
关键词:
Astilbe chinensis;bergenin;refluxing extraction;HPLC
摘  要:
Optimal conditions of extracting bergenin from Astilbe chinensis by reflux method were examined.Single factor experiments and orthogonal text were carried out to investigate the influences of the extraction factors on the yield of bergenin,which was measured by HPLC.The results showed that the best optimal conditions were solvent 45% ethanol,time of refluxing 2.5 hours,ratio of material to solvent 1∶20,and times of extraction 2.The yielding content of bergenin was 2.41% at the optimal conditions.

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