Position: Home > Articles > Antiosteoporotic Effects of Collagen Peptides and Zinc-Chelating Complex from Pearl Oyster Mantle
MEAT RESEARCH
2018
(2)
36-42+9
珍珠贝外套膜胶原蛋白肽及其锌螯合物的体外抑制骨质疏松作用
作 者：
马婷;吴谦;申铉日
单 位：
海南大学食品学院
关键词：
珍珠贝外套膜;肽锌螯合物;MC3T3-E1细胞;骨质疏松症
摘 要：
研究珍珠贝外套膜胶原蛋白肽(pearl oyster mantle collagen peptide,POM-CP)及其锌螯合物(zincchelating pearl oyster mantle collagen peptide,POMCP-Zn)对小鼠前成骨细胞MC3T3-E1增殖分化的影响。结果表明:噻唑蓝检测结果显示,与空白对照组相比,POMCP-Zn能够显著促进成骨细胞的增殖,而POM-CP对成骨细胞的增殖性无显著影响;与空白对照组相比,400μg/mL的POM-CP和10μg/mL的POMCP-Zn将成骨细胞的碱性磷酸酶活性分别提高到1.31倍和1.48倍;POMCP-Zn还可以提高成骨细胞分化阶段Ⅰ型胶原蛋白、骨钙素蛋白的分泌量,同时促进成骨细胞矿化阶段骨结节的形成。综上所述,与POM-CP相比,POMCP-Zn对成骨细胞MC3T3-E1的增殖和分化有较强的促进作用,POMCP-Zn有望成为预防骨质疏松症的潜在功能性食品。
译 名：
Antiosteoporotic Effects of Collagen Peptides and Zinc-Chelating Complex from Pearl Oyster Mantle
作 者：
MA Ting;WU Qian;SHEN Xuanri;College of Food Science and Technology, Hainan University;
关键词：
pearl oyster mantle;;zinc-chelating peptides;;MC3T3-E1 cells;;osteoporosis
摘 要：
This study was aimed to investigate the effects of peptides(POM-CP) and zinc-chelating peptides(POMCP-Zn) from pearl oyster mantle collagen on the proliferation and differentiation of the osteoblastic cell line MC3 T3-E1. The results of methyl thiazolyl tetrazolium assay(MTT) showed that POMCP-Zn significantly stimulated osteoblastic proliferation in comparison to the control, while POM-CP had no effect on cell proliferation. In addition, POM-CP at 400 μg/m L and POMCP-Zn at 10 μg/m L increased alkaline phosphatase activity 1.31-and 1.48-folds in osteoblastic cells compared with the control. Moreover, POMCP-Zn promoted the production of collagen type I and osteocalcin during osteoblastic differentiation and the formation of bone nodules osteoblastic mineralization. In conclusion, POMCP-Zn exerted a stronger effect than POM-CP on the proliferation and differentiation of MC3 T3-E1 and POMCP-Zn is a potential candidate functional factor for osteoporosis prevention.
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