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Position: Home > Articles > Study on HPLC-FPS of Saponins from Pairs polyphylla Smith var yunnanensis(Franch)Hara Journal of Anhui Agricultural Sciences 2009,37 (21) 164-165+514

滇重楼皂苷部位HPLC指纹图谱的研究

作  者:
张海珠;周浓;夏从龙
单  位:
大理学院药学院
关键词:
滇重楼;HPLC;皂苷;指纹图谱;质量评价
摘  要:
[目的]建立滇重楼皂苷有效部位HPLC指纹图谱分析方法,研究不同产地滇重楼药材的质量。[方法]采用HPLC等度洗脱的方法进行色谱分离,使用"相似度评价软件"进行数据处理,对不同产地的滇重楼药材皂苷部位进行指纹图谱的研究。[结果]确立了滇重楼药材皂苷部位指纹图谱的高效液相分析条件,确定8个共有峰。不同产地的滇重楼药材皂苷部位指纹图谱有一定差异。[结论]采用HPLC法制定滇重楼药材皂苷部位的指纹图谱,其皂苷类成分得到较好的分离,方法重现性好,可用于滇重楼的质量评价。
译  名:
Study on HPLC-FPS of Saponins from Pairs polyphylla Smith var yunnanensis(Franch)Hara
作  者:
ZHANG Hai-zhu et al(College of Pharmacy,Dali University,Dali,Yunnan 671000)
关键词:
Pairs polyphylla Smith var yunnanensis(Franch)Hara;HPLC;Saponins;Chromatography fingerprint;Quality assessment
摘  要:
[Objective] The research aimed to establish a method of HPLC-fingerprint spectrum(HPLC-FPS) for the active part in Pairs polyphylla Smith var yunnanensis(Franch)Hara in order to control it's quality from different palaces.[Method] The isocratic elution mode was applied in chromatographic separation,and data were analysed by "Similarity Evaluation software" to compare it's HPLC-FPS from different places.[Result] The conditions for HPLC analysis of Pairs polyphylla Smith var yunnanensis(Franch)Hara were established and 8 common peaks were separated.[Conclusion] All components in the spectrum were separated well and the HPLC fingerprint method is repeatable.The method can be used in quality assessment of Pairs polyphylla Smith var yunnanensis(Franch)Hara

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