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当前位置: 首页 > 文章 > 高氰和低氰木薯生氰葡萄糖苷酶Linamarase基因的组织表达差异分析 中国农学通报 2016,32 (11) 22-26
Position: Home > Articles > Differential Expression of Linamarase Gene in Cassava Cultivars with Low Cyanide Content and High Cyanide Content Chinese Agricultural Science Bulletin 2016,32 (11) 22-26

高氰和低氰木薯生氰葡萄糖苷酶Linamarase基因的组织表达差异分析

作  者：
付莉莉;沈培峰;谭德冠;孙雪飘;张家明
单  位：
中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室
关键词：
木薯;亚麻仁苷酶;生氰糖苷;RT-PCR;高氰
摘  要：
旨在降低或去除木薯的生氰糖苷,获得安全无毒木薯新品种。本研究以高氰和低氰5个品种的木薯为材料,通过RT-PCR方法,研究了生氰葡萄糖苷酶基因Linamarase在木薯中的表达情况。木薯Linamarase基因只在地上部分表达;5个木薯品种,包括低氰和高氰品种,在块根中也均未检测到Linamarase基因的表达。在地上部分中,顶芽的表达量要远远大于茎和成熟叶片的表达量,而且低氰和高氰品种在顶芽的表达量基本相同,但是低氰品种茎及成熟叶片中Linamarase的表达量比高氰品种相对较高。木薯生氰糖苷酶Linamarase在不同木薯品种不同组织的表达情况的不同,可以为筛选安全无毒木薯品种提供一个参考。
译  名：
Differential Expression of Linamarase Gene in Cassava Cultivars with Low Cyanide Content and High Cyanide Content
作  者：
Fu Lili;Shen Peifeng;Tan Deguan;Sun Xuepiao;Zhang Jiaming;Institute of Tropical Bioscience and Biotechnology, MOA Key Laboratory of Tropical Crop Biology and Genetic Resources, Chinese Academy of Tropical Agricultural Sciences;
关键词：
cassava;;linamarase;;cyanogenic glycoside;;RT-PCR;;high cyanide
摘  要：
Cyanogenic glucosides were reduced or eliminated in order to obtain safe and non-toxic cassavavarieties. In this study, the expression of a cyanogenic glucosidase gene was investigated in five cassavavarieties(high-cyanide and low-cyanide) by RT-PCR. Linamarase was synthesized in the aboveground tissuesof the plant and no expression was found in the underground part; the expression level of terminal bud on theaboveground part was much greater than the expression level of stems and mature leaves; no expressionappeared in tuberous root of low cyanide and high cyanide varieties. No significant difference in expression wasfound on the terminal bud of high cyanide and low-cyanide cassava; however, higher expression appeared inleaves and stems of low cyanide cassava. The expression of linamarase in different varieties and differenttissues of cassava was different. The study could provide certain reference for safe and non-poisonous cassavavariety screening.

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