Primary Study on the Biological Characteristics of Hypocrella bambusae Producing Red Pigment

LIU Wei et al (College of Science, Honghe University, Mengzi, Yunnan 661100)

Hypocrella bambusae; Biological characteristics; Medium

[Objective] The aim of this study was to enhance hypocrellin yield. [Method] The Hypocrella bambusae strains producing red pigment were isolated from wild H. bambusae for liquid culture to study the suitable medium, carbon (C) source, nitrogen (N) source and pH value for H. bambusae growth. [Result] H. bambusae grew fastest and excreted most pigment with thickest color on beef extract medium. Among treatments with 5 kinds of tested C sources, H. bambusae grew fastest on lactose medium with most excreted pigment and secondary maltose, D-xylose and soluble starch. The influences of different N sources on H. bambusae growth had obvious difference. The growth velocity of H. bambusae was fastest on NH4NO3 medium with most excreted pigment and its growth potential was slowest on NH4Cl medium. The influences of different pH values on H. bambusae growth were more obvious. H. bambusae could grow in the pH value of 3~9. When pH value was 6, the growth potential of H. bambusae was best with most excreted pigment. [Conclusion] The growth velocity of H. bambusae was fastest on beef extract medium. The C source and N source, which was easiest to utilize, were lactose and NH4NO3. It could grow when pH value was 3～9 and the pH value of 6 was most suitable.