作 者：
周光斌;郭将;曾艳;程柯仁;傅祥伟;朱士恩
单 位：
中国农业大学动物科技学院;四川农业大学动物科技学院动物遗传育种研究所
关键词：
Oct-4;超低温冷冻;桑椹胚;昆明白小鼠
摘 要：
本实验旨在探讨昆明白小鼠桑椹胚超低温冷冻后Oct-4表达的变化与胚胎发育潜力的关系。胚胎采用OPS法冷冻,即胚胎于10%EG+10%DMSO溶液中预处理30 s,然后再移入玻璃化溶液(EDFS30)中处理25 s,以OPS为承载器投入液氮中。毒性组胚胎未投入液氮,其他过程与冷冻组相同,新鲜胚胎为对照组。胚胎解冻后,检测Oct-4 mRNA与蛋白的表达,通过观察其形态正常率、囊胚发育率和囊胚细胞数来综合判断其体外发育能力。结果表明:冷冻组和毒性组较桑椹胚中Oct-4 mRNA的表达量对照组相比显著降低(P<0.05),蛋白表达量3组间无显著差异。桑椹胚处理后的形态正常率以及桑椹胚培养48 h后的囊胚发育率(96.67%～100%)和囊胚细胞数(89.67～92.33)3组间无显著差异。结果显示,玻璃化冷冻保存小鼠桑椹胚后多能性基因Oct-4 mRNA表达的变化并未影响其体外发育潜力。
译 名：
Oct-4 Expression in Mouse Morula after Cryopreservation
作 者：
ZHOU Guang-bin1,2,GUO Jiang1,ZENG Yan2,CHENG Ke-ren2,FU Xiang-wei2,ZHU Shi-en2(1.Institute of Animal Genetics and Breeding,College of Animal Science and Technology,Sichuan Agricultural University,Sichuan Chengdu 611130,China;2.College of Animal Science and Technology, China Agricultural University,Beijing 100193,China)
关键词：
oct-4;cryopreservation;morula;Kunming white mous
摘 要：
This study was conducted to investigate the effect of cryopreservaion on oct-4 expression and the in vitro development of Kunming mouse morula.The morulas were randomly allocated into three groups: untreated(control),exposed to vitrification solution without being plunged into liquid nitrogen(toxicity),or vitrified by open-pulled straw method(vitrification).After vitrification and warming,the embryos were morphologically evaluated and assessed by their development to blastocysts,or by the average cell number of blastocyst.Oct-4 expression in morula was assessed by qRT-PCR and western blotting.The results showed that the oct-4 mRNA in toxicity and vitrification groups were significantly lower(P<0.05) than that of control.However,there was no significant difference in oct-4 expression at protein level among three groups.The blastocyst rate(96.67% to 100.00%) and the average cell number of blastocyst(89.67 to 92.33) were similar among them.The data demonstrated that the decreased oct-4 mRNA due to cryopreservation didn'tnegatively affect the in vitro developmental potential of mouse morula.
