当前位置: 首页 > 文章 > 枸杞品种SSR荧光指纹图谱构建及遗传关系分析 西北林学院学报 2017,32 (1) 137-141
Position: Home > Articles > Fingerprinting and Genetic Diversity Analysis of Wolfberry Cultivars Using Fluorescence-labeled SSR Markers Journal of Northwest Forestry University 2017,32 (1) 137-141

枸杞品种SSR荧光指纹图谱构建及遗传关系分析

作  者:
尹跃;安巍;赵建华;李彦龙;樊云芳;曹有龙
单  位:
宁夏农林科学院国家枸杞工程技术研究中心
关键词:
枸杞;SSR;DNA指纹图谱;遗传多样性
摘  要:
以12个枸杞栽培品种为材料,利用SSR荧光标记进行DNA指纹图谱的构建和遗传关系分析。11对SSR引物在12品种共检测到34个等位变异,平均每个位点为3.1个,位点平均有效等位变异数(Ne)、观测杂合度(Ho)、Shannon’s信息指数(I)和多态信息含量(PIC)分别为2.025、0.439、0.776和0.391。其中4对引物SF1、SF30、SF63和SF92可区分所有供试品种,为品种鉴别提供依据。NTSYS-pc2.10软件聚类表明,12个枸杞品种遗传相似系数变化范围是0.57~0.91,平均为0.68,表明枸杞品种间存在着丰富的遗传多样性。SSR荧光标记技术可以有效地用于枸杞品种资源指纹图谱构建及遗传关系研究。
译  名:
Fingerprinting and Genetic Diversity Analysis of Wolfberry Cultivars Using Fluorescence-labeled SSR Markers
作  者:
YIN Yue;AN Wei;ZHAO Jian-hua;LI Yan-long;FAN Yun-fang;CAO You-long;Ningxia Academy of Agriculture and Forestry Sciences,National Wolfberry Engineering Research Center;
关键词:
wolfberry;;SSR;;DNA fingerprinting;;genetic diversity
摘  要:
Twelve wolfberry cultivars were used as materials to establish DNA fingerprinting and analyzed genetic diversity with simple sequence repeat(SSR)fluorescent marker.A total of 34 alleles were detected by 11 SSR polymorphic markers,with an average of 3.1alleles per locus.The values of Ne,Ho,Iand PIC were 2.025,0.439,0.776,and 0.391,respectively.SF1,SF30,SF63,and SF92 could distinguish all the cultivars.NTSYS-pc 2.10 software was used to analyze genetic diversity of 12 wolfberry cultivars.The results indicated that genetic similarity coefficients of 12 cultivars ranged from 0.57 to 0.91 with an average of0.68,which showed that there was abundant genetic diversity in wolfberry cultivars.Fluorescence-labeled SSR markers could effectively conduct fingerprinting and genetic diversity analysis for wolfberry cultivars.

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