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Position: Home > Articles > Influence on callus induced by Hormone of Maackia amurensis Rupr. Forest Investigation Design 2012 (3) 77-78

激素对怀槐愈伤组织诱导的影响

作  者:
王志勇
单  位:
黑龙江省第三森林调查规划设计院
关键词:
怀槐;组织培养;激素;异黄酮
摘  要:
在怀槐组织培养中,通过对比不同激素浓度对愈伤组织诱导效果及异黄酮分泌量的影响程度,分析出最佳的激素种类及使用浓度。研究发现:(1)怀槐的最佳愈伤组织诱导激素为:NAA 0.5mg/L、2,4-D 2.5mg/L、6BA 1.5mg/L、KT 0mg/L,激素NAA对愈伤组织诱导的影响程度最大;(2)怀槐愈伤组织中异黄酮分泌的最优质诱导激素为:激素NAA和6BA,当浓度分别为0.5mg/L和1.5mg/L时,能够使愈伤组织的异黄酮分泌量达到最高。
译  名:
Influence on callus induced by Hormone of Maackia amurensis Rupr.
作  者:
Wang Zhiyong(The third Forest Inventory and Planning Academy of Heilongjiang Province,152000 Suihua, Heilongjiang,China)
关键词:
Maackia amurensis Rupr.;Tissue Culture;Hormone;Isoflavone
摘  要:
In tissue culture of Maackia amurensis Rupr.,through the comparison of different hormone concentrations on callus induction effect and the degree of influence of the amount of isoflavones secretion,analyze the best type of auxin and the use of concentration,the study found:(1) Maackiahormone concentration for callus induction: NAA 0.5mg / L,2,4-D,2.5mg / L,the of 6BA 1.5mg / L,KT mg / L of NAA on the degree of callus induction;(2)Maackia amurensis callus isoflavones secreted in the highest quality induced by hormones as follows: Effect of NAA and 6BA,when the highest concentrations were 0.5mg / L and 1.5mg / L,enabling the isoflavone secretion of callus reached.

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