中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 3株H1N1亚型猪流感病毒的HA基因遗传信息及抗原特异性分析 华南农业大学学报 2017 (5) 13-18
Position: Home > Articles > Analysis of genetic information in HA genes and antigen specificities of three strains of H1N1 swine influenza virus Journal of South China Agricultural University 2017 (5) 13-18

3株H1N1亚型猪流感病毒的HA基因遗传信息及抗原特异性分析

作  者：
秦锋;蔡孟楷;张伟东;张京;方博;黄俊明;卜德新;罗勇峰;马骏;付新亮;曹振鹏;张桂红
单  位：
华南农业大学医院;烟台市福山区动物卫生监督所;广东省动物源性人兽共患病预防与控制重点实验室/国家生猪种业工程技术研究中心/华南农业大学兽医学院
关键词：
猪流感病毒;H1N1;遗传信息;抗原表位;血凝抑制;抗体滴度;HA基因
摘  要：
【目的】分析3株属于欧亚类禽(SWSS1)、经典(SWL6)与Pdm09H1N1(Pdm091057)分支的猪流感病毒的HA基因遗传信息及抗原的特异性,为HA基因抗原表位的功能研究及流感防控奠定基础。【方法】以SWSS1株、SWL6株和Pdm091057株流感病毒为材料,比较分析3株H1N1亚型HA基因片段的遗传信息,制备全病毒灭活疫苗,各免疫3只雌性新西兰大白兔,采用血凝抑制(Hemagglutination inhibition,HI)反应试验检测抗体滴度。【结果】3株病毒的HA基因片段的氨基酸序列相似性为69.4%~89.1%,各分支的HA基因的抗原表位存在差异;3株病毒经过2次免疫后平均HI抗体滴度均能达1 280以上,且SWSS1的平均HI抗体滴度高达2 560。同时SWSS1与SWL6、Pdm091057这2株病毒均无血清学交叉反应,而SWL6与Pdm091057有较低的血清学交叉反应。【结论】3株猪流感病毒的HA基因片段抗原表位存在着差异,可能是3毒株之间血清学交叉反应较低的原因。3株病毒免疫原性均较好,可作为候选疫苗株。
译  名：
Analysis of genetic information in HA genes and antigen specificities of three strains of H1N1 swine influenza virus
作  者：
QIN Feng;CAI Mengkai;ZHANG Weidong;ZHANG Jing;FANG Bo;HUANG Junming;BU Dexin;LUO Yongfeng;MA Jun;FU Xinliang;CAO Zhenpeng;ZHANG Guihong;Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/National Engineering Research Center for Breeding Swine Industry/College of Veterinary, South China Agricultural University;Hospital of South China Agricultural University;Animal Health Supervision of Fushan District;
关键词：
swine influence virus;;H1N1;;genetic information;;antigenic epitope;;hemagglutination inhibition;;antibody titer;;HA gene
摘  要：
【Objective】 To study the genetic information in HA genes and antigen specificities of SWSS1, SWL6 and Pdm091057 strains which respectively belongs to Eurasian avaian-like, classical and Pdm09H1N1 branches of H1N1 swine influenza viruses(SIV), and provide a basis for functional study on antigenic epitopes of HA gene as well asprevention and control of influenza virus.【Method】We compared the genetic information in HA gene fragments of three H1N1 subtypes, SWSS1, SWL6 and Pdm091057. Inactivated SIV vaccines were prepared and each type of vaccine was used to immune three New Zealand white rabbit. The hemagglutination inhibition(HI) reaction was used to detect antibody titers. 【Result】 The amino acid sequences of HA gene fragments had 69.4%-89.1% similarities among three SIV strains. There were variations in antigenic epitopes of HA genes of the three strains. The average HI antibody titers were all above 1 280 for three strains after the second immunization, and the average HI antibody titer for SWSS1 reached 2 560. SWSS1 had no serological cross reaction with SWL6 or Pdm091057 strain, while SWL6 and Pdm091057 strains had low degree of serological cross reaction. 【Conclusion】Three SIV strains have differences in antigenic epitopes of HA gene, which may be the reason for the low degree of serological cross reaction among strains. All three strains have good immunogenicity, and can be used as candidate vaccine strains.

相似文章

导出引用
计量
文章访问数: 3
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46