单 位：
中国农业科学院植物保护研究所/农业部生物防治重点开放实验室
关键词：
链孢粘帚霉;核盘菌;菌寄生;EST;寄生相关基因
摘 要：
链孢粘帚霉Gliocladium catenulatum是一种重要的菌寄生真菌,为了明确该菌与寄主核盘菌Sclerotinia sclerotiorum互作中参与菌寄生过程的功能基因,本实验室前期采用抑制消减杂交技术(SSH)构建了消减cD-NA文库。本文从该库里随机挑选420个阳性克隆进行测序分析,结果表明克隆片段大小在200~600bp的序列占81.07%。去除载体和小于100bp的序列,获得391条有效序列,其平均长度为409bp。通过序列拼接得到181个单值基因(unigenes),其中包括27个重叠群(contigs)和154个单拷贝的ESTs(singletons)。将得到的ESTs与NCBI非冗余蛋白数据库(NR)进行BLASTx分析,结果显示有38个基因的编码蛋白与已知功能的蛋白有较高的相似性,其中包括可能与生物防治功能相关的内切葡聚糖酶、脂滴包被蛋白、MFS转运蛋白、过氧化物酶等;有39条序列在NCBI数据库中未找到相似序列,可能为新基因;其他基因的编码蛋白与数据库中功能未知蛋白相似性高。本研究为明确与重寄生功能相关的基因奠定了基础。
译 名：
EST Bioinformatical Analysis of Mycoparasitism-associated Genes in Gliocladium catenulatum Parasitizing on Sclerotia of Sclerotinia sclerotiorum
作 者：
ZHONG Zeng-ming,LI Shi-dong*,GAO Hui-lan,SUN Man-hong (Key Laboratory for Biological Control of Ministry of Agriculture,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
关键词：
Gliocladium catenulatum;Sclerotinia sclerotiorum;mycoparasitism;EST;mycoparasitism-associated gene
摘 要：
Gliocladium catenulatum is an important mycoparasite.To explore the mycoparasitism-associated genes parasitizing on sclerotia of Sclerotinia sclerotiorum,a subtracted cDNA library by suppression subtractive hybridization(SSH) was constructed.In this paper,420 positive clones were sequenced by randomly selecting with PCR from the library.Analyses indicated that size of 81.07% inserts in all clones was between 200-600 bp.391 valid sequences with average size of 409 bp were generated by vector trimming and discarding of the sequences shorter than 100 bp.Initial assembly resulted in 181 unigenes,including 27 contigs and 154 singletons.BLASTx against a non-redundant protein sequence database in NCBI gave rise to 38 genes which were highly homologous with functional genes,including the genes encoding endoglucanase,perilipin-like protein,MFS transporter,peroxidase,etc,which were related to biocontrol.Another 39 gene fragments had no homology with the sequences deposited in NCBI,and were therefore supposed to be novel genes.Other genes were homologous with the genes encoding proteins with unknown functions in database.This research laid the foundation for finding genes that related to mycoparasitism.
