中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 马铃薯及其制品中转基因成分的多重PCR检测 食品科学 2006,27 (1) 178-181
Position: Home > Articles > Multiplex PCR Detection of Genetically Modified Components in Potato and Its Products FOOD SCIENCE 2006,27 (1) 178-181

马铃薯及其制品中转基因成分的多重PCR检测

作  者：
邵碧英;陈文炳;杨婕
单  位：
福建出入境检验检疫局
关键词：
马铃薯;转基因成分;多重PCR
摘  要：
采用CTAB法提取15个马铃薯及其制品样品中的总DNA,内源PATA基因的PCR扩增结果均为阳性,表明已提取到DNA。应用根癌农杆菌胭脂碱合成酶基因(nos)终止子和大肠杆菌K12菌株新霉素磷酸转移酶Ⅱ(nptⅡ)基因的二重PCR对样品进行转基因成分检测,结果均为阴性。将马铃薯DNA和阳性质粒pBI121混合作为PCR的反应模板,建立了内源PATA基因、花椰菜花叶病毒(Cauliflowermosaicvirus,CaMV)35S启动子、nos终止子和nptⅡ基因之间的多重PCR检测方法。多重PCR方法具有节约试剂、节省时间等特点,在转基因产品检测上的应用值得推广。
译  名：
Multiplex PCR Detection of Genetically Modified Components in Potato and Its Products
作  者：
SHAO Bi-ying,CHEN Wen-bing,YANG Jie (Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350003, China)
关键词：
potato;genetically modified component;multiplex PCR
摘  要：
The total DNAs of fifteen potatoes and their product samples were extracted by CTAB method. The PCR detection results of endogenous PATA gene were all positive, which showed that the DNAs were extracted successfully. The duplex PCR detection results of Agrobacterium tumefaciens nopaline synthase (nos) terminator and Escherichia coli strain K12 neomycin phosphotransferase II (nptⅡ) gene in these samples were all negative. The multiplex PCR detection methods of endogenous PAPA gene, Cauliflower mosaic virus (CaMV) 35S promoter, nos terminator and nptⅡ gene were developed, using the mixture of potato DNA and positive plamid pBI121 as PCR template. The multiplex PCR has some traits such as saving reagent and time, so it may be popularized to detect genetically modified products.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46