中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 双水相–SPE–TFA–GC/MS测定烟草中糖苷类潜香物质方法的建立 湖南农业大学学报(自然科学版) 2020 (3) 291-296
Position: Home > Articles > Determination of glycosides in tobacco by aqueous two-phase-SPE-TFA-GC/MS Journal of Hunan Agricultural University(Natural Sciences) 2020 (3) 291-296

双水相–SPE–TFA–GC/MS测定烟草中糖苷类潜香物质方法的建立

作  者：
彭振兴;郭东锋;王正刚;姚忠达;张福建;牛慧伟
单  位：
关键词：
烟草;糖苷;固相萃取;衍生化;气相色谱–质谱
摘  要：
建立了双水相–固相萃取(SPE)–三氟乙酰衍生化(TFA)–GC/MS测定烟草中糖苷类潜香物质的方法:烟草样品通过微波双水相提取、0.45μm膜过滤后得到的糖苷粗提物,过HP–20 SPE小柱,再依次经过正戊烷–二氯甲烷和甲醇洗脱,洗脱液经浓缩至干燥后得到的糖苷精提物,通过三氟乙酰胺(MBTFA)衍生化,采用葡萄糖苷标准物及NIST谱库比照定性,并与内标2–硝基苯–β–D–葡萄糖苷的衍生化产物面积比进行定量分析,可测定烟草中18种糖苷类潜香物质。利用该方法测定烤烟K326、白肋烟Kenek、香料烟Basma的糖苷类潜香物质,共测得18种糖苷类香味成分,糖苷包括14种葡萄糖苷、1种芸香糖苷和3种巢菜糖苷,苷元包括脂肪醇、芳香族化合物、降异戊二烯类、倍半萜类多酚类化合物;烤烟中糖苷类潜香物含量最高,香料烟中含量最低。
译  名：
Determination of glycosides in tobacco by aqueous two-phase-SPE-TFA-GC/MS
作  者：
PENG Zhenxing;GUO Dongfeng;WANG Zhenggang;YAO Zhongda;ZHANG Fujian;NIU Huiwei;China Tobacco Anhui Industrial Co.Ltd;Staff Development Institute of China National Tobacco Corporation;
关键词：
tobacco;;glycoside;;solid-phase extraction;;derivatization;;gas chromatography-mass spectrometer
摘  要：
Aqueous two-phase solid-phase extraction(SPE)-TFA trifluoroacetylation(TFA)-GC/MS was developed for determination of glycosides in tobacco. The sample was extracted by microwave two-phase extraction, and separated by 0.45 μm membrane, and then by HP-20 SPE column, which was eluted by N-pentane dichloromethane and methanol, the eluent was collected, concentrated and dried to obtain the glycoside extract. The acetylation of trifluoroacetamide(MBTFA) was used as a derivatization method, the quality of the derivative was compared with the certified reference material of glucoside and the NIST spectrum library, and the area ratio of the derivative to the internal standard of 2-nitrobenzene-β-D-glucoside was quantitatively analyzed, by which 18 glycosides in tobacco were identified. The method was applied to the determination of flue-cured tobacco(K326), burley tobacco(Kenek) and oriental tobacco(Basma), and 18 glycoside flavor components were identified. The glycoside types include 14 glucosides, 1 rutin, and 3 nesteosides; and the aglycone types include aliphatic alcohols, aromaticity, norisoprenoids, sesquiterpenoids, and polyphenols. The content of glycosides in flue-cured tobacco was the highest, and in oriental tobacco the lowest.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46