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当前位置: 首页 > 文章 > 2006-2007年陕西省古典猪瘟流行毒株E0基因的克隆及序列分析 浙江大学学报(农业与生命科学版) 2010 (1) 16-22
Position: Home > Articles > CLONING AND SEQUENCE ANALYSIS OF E0 GENE OF VIRULENT CLASSICAL SWINE FEVER VIRUS ISOLATES IN SHAANXI PROVINCE IN 2006-2007 Journal of Zhejiang University (Agriculture and Life Sciences) 2010 (1) 16-22

2006-2007年陕西省古典猪瘟流行毒株E0基因的克隆及序列分析

作  者：
吴旭锦;朱小甫;陈德坤
单  位：
西北农林科技大学动物医学院;咸阳职业技术学院生物科技系
关键词：
古典猪瘟;E0基因;克隆;序列分析
摘  要：
根据GENBANK上发表的古典猪瘟参考毒株SHIMEN毒株全基因组序列,设计并合成2对引物,采用巢式RT-PCR技术,从陕西省猪病料中成功扩增出9株猪瘟流行毒株E0全基因并测定其核苷酸序列,与已发表的ALD、ALFORT187、BRESCIA、C HVRI、CAP、SHIMEN、GXWZ02和HCLV等代表毒株进行同源性比较分析.核苷酸分析表明:这9株猪瘟流行毒株可以分为2大群,1株(LN163)属于群 I,与SHIMEN强毒株、HCLV疫苗株和GXWZ02野毒株等代表株的同源性为80.8%~82.4%;另外8株属于群 II,毒株之间E0基因核苷酸序列的同源性为93.6%~99.6%.氨基酸序列分析表明:9株流行毒株中,群 I中的LN163毒株与我国野毒参照株GXWZ02的同源性只有83.9%,而其余8株与GXWZ02株氨基酸序列的同源性为94.4%~98.1%,形成明显不同的进化分支;同时这9株流行毒株与我国疫苗株HCLV和经典强毒株SHIMEN的氨基酸序列同源性分别为88.0%~89.5%和89.1%~91.8%,均呈现较明显的变异.
译  名：
CLONING AND SEQUENCE ANALYSIS OF E0 GENE OF VIRULENT CLASSICAL SWINE FEVER VIRUS ISOLATES IN SHAANXI PROVINCE IN 2006-2007
关键词：
CLASSICAL SWINE FEVER%E0 GENE%CLONING%SEQUENCE ANALYSIS
摘  要：
According to the published genetic sequences of reference strain Shimen on GenBank, two pairs of primers were synthesized to amplify the E0 gene of nine prevalent virulent classical swine fever virus(CSFV) isolates from swine samples in Shaanxi Province by reverse transcription-nested polymerase chain reaction (nested RT-PCR). The nucleotide and amino acid sequences of these isolates were also compared and analyzed with reference strains ALD, Alfort187, Brescia, C HVRI, CAP, Shimen, GXWZ02 and HCLV. The nucleotide analysis showed that these nine isolates could be divided into two groups, one of them(LN163) belonged to group I sharing 80.8%-82.4%homology, while the other eight isolates belonged to group II sharing 93.6%-99.6%homology with reference strains within respective groups. Amino acids sequences analysis indicated that these nine isolates developed two different evolving branches, the strain LN163 of which shared 83.9%homology and the other eight isolates shared 94.4%-98.1%homology compared with the reference wild strain GXWZ02 from China, and these nine field isolates had obvious variation for the 88.0%-89.5%and 89.1%-91.8%homology of amino acids sequences compared with HCLV and Shimen, respectively.

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