Position: Home > Articles > Preparation of Ag@SiO_2 Nanoparticles and Detection of Adenosine by Light Scattering
Journal of Southwest University(Natural Science Edition)
2017
(9)
113-117
硅包银纳米颗粒的制备及其光散射法检测腺苷
作 者：
李靖云;韩国斌;刘建萍;李原芳
单 位：
发光与实时分析教育部重点实验室/西南大学化学化工学院;重庆第48中学
关键词：
硅包银纳米颗粒;磁性颗粒;光散射;腺苷;核酸适配体
摘 要：
将腺苷的核酸适配体设计成两段DNA链,一段修饰在硅包银纳米颗粒上,另一段修饰在磁性颗粒上.利用腺苷与其核酸适配体的特异性结合,通过检测磁性分离后上清液中硅包银纳米颗粒的光散射信号变化,实现了腺苷检测.方法的线性范围为8.0×10~(-6)~5.0×10~(-4)mol/L,线性相关系数(r)为0.981 8,其他的核苷不干扰测定.
译 名：
Preparation of Ag@SiO_2 Nanoparticles and Detection of Adenosine by Light Scattering
作 者：
LI Jing-yun;HAN Guo-bin;LIU Jian-ping;LI Yuan-fang;Education Ministry Key Laboratory on Luminescence and Real-Time Analysis/School of Chemistry and Chemical Engineering,Southwest University;Chongqing Forty-Eighth Middle School;
关键词：
Ag@SiO2 nanoparticle;;magnetite particle;;light scattering;;adenosine;;aptamer
摘 要：
Silver nanoparticles(AgNPs)have the property of strong localized surface plasmon resonance scattering,but their stability and functionality are inferior.To solve these problems,silica-coated silver(Ag@SiO_2)nanoparticles were prepared by packing a SiO_2 layer on AgNPs.Then a new method was designed for adenosine detection by using Ag@SiO_2NPs with separation of magnetic particles(MPs).It was found that a linear relationship(ΔILS=666.50+505.18logc)was obtained between the value ofΔILSand the concentration of adenosine in the range of 5.0×10~(-4)-8.0×10~(-6) mol/L with a correlation coefficient(r) of 0.981 8.Moreover,no obvious effects were detected of other nucleosides on the analysis of adenosine.
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