Establishment of a fibroblast cell line with 13 /17 Robertsonian translocation pig

MA Kang;QI Shun-min;YU Guang-hui;SUN Jin-hai;College of Animal science and Technology,Qingdao Agricultural University;

13 /17 Robertsonian translocation pig;;fibroblast cell line;;cell isolation;;karyotype analysis;;biological characteristics

To study the germplasm resource of 13 /17 Robertsonian translocation in pig populations at the cellular level,a method for the isolation of tissue blocks was adopted to isolate and culture the ear tissues from 13 /17 Robertsonian translocation in heterozygous pigs,homozygous pigs,and normal karyotype pigs. Three fibroblast cell lines were established,and used to analyze the biological characteristics,including the morphology,growth kinetics,karyotype analysis,transfection of green fluorescent protein reporter plasmid,microbial contamination detection,and so on. The results showed that the cell morphology of the pigs with three kinds of karyotypes was all standard fibroblast morphology,and the intracellular vimentin presented positive expression,and the growth curve was sigmoidal. The proportion of 2n = 37 chromosomes in heterozygous pigs was 92. 5%,the proportion of 2n = 36 chromosomes in homozygous pigs was 93. 8%,and the proportion of 2n = 38 chromosomes in96. 3% in normal karyotype pigs. Exogenous plasmids were stably expression in the cells. The detection of bacteria,fungi,viruses,and mycoplasma were negative. The results indicate that a fibroblast cell line of 13 /17 Robertsonian translocation pigs is successfully established.