Position: Home > Articles > Determination of Protein Content in Reconstituted Tobacco Coating Liquid by Coomassie Brilliant Blue Method
Hubei Agricultural Sciences
2017,56
(5)
946-947+950
考马斯亮蓝法对烟草薄片涂布液中蛋白质含量的测定
作 者：
杨静;白冰;王宁;张改红
单 位：
河南卷烟工业烟草薄片有限公司;郑州轻工业学院食品与生物工程学院
关键词：
薄片涂布液;蛋白质;考马斯亮蓝法
摘 要：
采用考马斯亮蓝法对烟草薄片涂布液中的蛋白质含量进行了测定,建立了快速、灵敏的蛋白质检测方法。结果表明,蛋白质含量在14~140μg/m L与吸光度呈良好的线性关系(R2=0.999 5),样品平均回收率为101.5%,RSD为1.4%。该方法简便、快速、准确度高、重现性好,可以作为烟草薄片涂布液及烟草料液中蛋白质测定的有效方法。
译 名：
Determination of Protein Content in Reconstituted Tobacco Coating Liquid by Coomassie Brilliant Blue Method
作 者：
YANG Jing;BAI Bing;WANG Ning;ZHANG Gai-hong;School of Food and Bioengineering,Zhengzhou University of Light Industry;Henan Cigarette Industry Tobacco Sheet Co.Ltd.;
关键词：
reconstituted tobacco coating liquid;;protein;;coomassie brilliant blue method
摘 要：
A rapid and sensitive Coomassie brilliant blue method was developed for protein determination in reconstituted tobacco coating liquid. The results showed that,the protein content had a good linear relationship with the absorbance in the range of14~140 μg/m L(R2=0.999 5). The average recovery was 101.5% and RSD was 1.4%. The method was simple,rapid,accurate and have high reproducibility,which could be used to determine the protein content in reconstituted tobacco coating liquid and tobacco sauce as an efficient method.
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