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Journal of Yangzhou University(Agricultural and Life Science Edition)
2017,38
(3)
62-67
乳酸菌和酵母菌混合发酵脱毒橡胶籽的安全性试验研究
作 者：
彭塘福;陈晗;敬伟甫;林连兵;邓先余
单 位：
昆明理工大学生命科学与技术学院
关键词：
融合魏斯菌;库德里阿兹威毕赤酵母;橡胶籽;混合发酵
摘 要：
选昆明小鼠130只,雌雄各半,随机分为13组,每组10只。7d灌胃急性毒性试验和30d灌胃慢性毒性试验各5组,30d饲喂试验3组,通过灌胃的给药方式测量每组小鼠的体重和脏器比,以验证融合魏斯菌(Weissella confusa)CMR-1与库德里阿兹威毕赤酵母(Pichia kudriavzevii)JM-2的安全性以及评估混合发酵脱毒橡胶籽的安全性。结果表明:在7d急性毒性试验及30d慢性毒性试验中,灌胃CMR-1、JM-2与对照组小鼠相比均无显著差异;在30d饲喂试验中,饲喂脱毒橡胶籽的小鼠与对照组相比无显著差异。饲喂未发酵脱毒橡胶籽的小鼠与对照组相比,心体比、肝体比出现极显著差异,脾体比、肺体比、肾体比无显著性差异。
译 名：
Experimental study on the safety of detoxified rubber seeds mixedly fermentated by lactic acid bacteria and yeast
作 者：
PENG Tangfu;CHEN Han;JING Weifu;LIN Lianbing;DENG Xianyu;Coll of Life Sci and Tech,Kunming Univ of Sci and Tech;Kunming Eco-Food Biot Ltd;
单 位：
Coll of Life Sci and Tech,Kunming Univ of Sci and Tech%Kunming Eco-Food Biot Ltd
关键词：
Weissella confusa;;Pichia kudriavzevii;;rubber seed;;mixed fermentation
摘 要：
130 Kunming rats were randomly divided into 13 groups,and each group had ten rats.5 groups were arranged in 7 dacute toxicity tests and 30 dchronic toxicity tests resepectively,and 3 groups were 30 dfeeding tests.The body weight and organ ratio of each group mice were measured by gavage administration.The objectives of the study were to verify the safety of Weissella confusa and Pichia kudriavzevii,and to evaluate the safety of detoxified rubber seeds which were mixedly fermentated.During the 7 dacute toxicity tests and 30 dchronic toxicity tests,the body weight and organ ratio of mice by feeding strain CMR-1,JM-2 compared with the MRS control group,YPD control group and normal control group were not significantly different(P>0.05).Organ ratio of mice that were fed detoxified rubber seeds compared with control groups showed no significant difference(P>0.05)in the 30 dfeeding tests.The heart and weight ratio,liver and weight ratio of mice which fed with no fermentated rubber seeds compared with that of control group,had a significant difference(P<0.01),and the spleen weight ratio,lung weight ratio and kidney weight ratio had no significant difference(P>0.05).
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