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Position: Home > Articles > Effect of NaCl autolysis promoter on the ceramids content of Candida lipolytica Sichuan Animal & Veterinary Sciences 2005,32 (9) 34-35

食盐助溶剂对解脂假丝酵母神经酰胺含量的影响

作  者:
赵静;陈国强;周全;林会兰
单  位:
青海大学农牧学院;清华大学生物科学与技术系
关键词:
神经酰胺;固相萃取法;薄层色谱;高效液相色谱-蒸发光散射器
摘  要:
用食盐作为助溶剂采用细胞自溶法,对解脂假丝酵母33MCandida lipolytica细胞壁进行破碎后提取神经酰胺,用SPE(固相萃取法)将神经酰胺与膜中其它脂类分离,分别应用TLC(薄层色谱)方法和HPLC-ELSD(高效液相色谱-蒸发光散射器)对其进行定性和定量分析,取得了良好效果。
译  名:
Effect of NaCl autolysis promoter on the ceramids content of Candida lipolytica
作  者:
ZHAO Jing1,CHEN Guo-qiang2, et al.(1.College of Agriculture and Husbandry in Qinghai University,Qinghai Xining 810003,China;2.Department of Biologic Technology in Qinghua University,Beijing 100008,China)
关键词:
ceramids; SPE; TLC; HPLC-ELSD
摘  要:
NaCl autolysis promoter was applied to destroy cell wall for Candida lipolytica ceramids extraction.In order to acquire better chromatography result and protect the elution column,SPE was applied to isolate ceramids from another membrane lipids.TLC and HPLC-ELSD were applied for qualitative and quantitative analysis respectively.

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