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Position: Home > Articles > Immunological characteristics of Anti-PrP McAbs Journal of Yangzhou University(Agricultural and Life Science Edition) 2009,30 (4) 9-13

抗朊蛋白单克隆抗体的免疫学特性研究

作  者:
吴晓东;张永强;刘雨田;邹艳丽;刘珊;李林;王志亮
单  位:
扬州大学农业部畜禽传染病学重点开放实验室;中国动物卫生与流行病学中心-国家外来动物疫病诊断中心-国家牛海绵状脑病参考实验室
关键词:
传染性海绵状脑病;朊蛋白;单克隆抗体;免疫学特性
摘  要:
以牛、羊重组朊蛋白(PrP)、正常牛、羊的脑组织匀浆、牛海绵状脑病(BSE)及羊痒病阳性的脑组织匀浆及切片为抗原,测定9株抗朊蛋白单克隆抗体的ELISA、免疫印迹及免疫组化等免疫学反应特性。结果表明:5株单抗可用于BSE及羊痒病的ELISA、免疫印迹检测;3株单抗可用于BSE及羊痒病的免疫组化检测;1株单抗在免疫印迹试验中只与羊痒病PrPSc的核心片段反应,结合该株单抗所识别的抗原表位PrP第78~93氨基酸(aa),证实BSE与羊痒病PrPSc的蛋白酶K消化位点存在差异。
译  名:
Immunological characteristics of Anti-PrP McAbs
作  者:
WU Xiao-dong1,2,ZHANG Yong-qiang2,LIU Yu-tian2,ZOU Yan-li2,LIU Shan2,LI Lin2,WANG Zhi-liang2(1.Key Lab of Anim Inf Dis of MOA,Yangzhou Univ,Yangzhou 225009,China;2.Nat BSE Ref Lab,Nat Diag Cen for Exotic Ani Dis,CAH and EC,Qingdao 266032,China)
关键词:
TSEs;Prion Protein(PrP);monoclonal antibody(McAb);immunological characteristics
摘  要:
In recent years,a series of anti-PrP monoclonal antibodies(McAbs) had been developed in our lab.In this research,9 McAbs immuno-reactivities with PrP were tested in ELISA,Western blotting and immunohistochemistry(IHC) assay.The results indicated that 5 McAbs could be used for the detection of BSE and Scrapie in Western blotting and ELISA,and 3 McAbs could be used for the detection of BSE and Scrapie in IHC assay.1 McAb only recognized the core part of PrPSc from sheep in Western blotting,and its epitope was located in 78-93aa of PrP.Therefore,it could differentiate the proteinase K cleavage site of PrPSc from BSE and Scrapie.

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