Position: Home > Articles > Ultrasonic-assisted Extraction and Antioxidant Activity of Polysaccharides From Laccaria laccata
Northern Horticulture
2018
(17)
133-138
红蜡蘑多糖的超声波辅助提取工艺及其体外抗氧化性研究
作 者：
张水花;李本鸿;周伟东
单 位：
曲靖师范学院化学与环境科学学院;曲靖师范学院图书馆
关键词：
红蜡蘑;多糖;超声波;DPPH·;ABTS+·
摘 要：
以红蜡蘑为试材,研究了液料比、超声功率、超声时间、超声温度、提取次数对多糖得率的影响,在单因素试验基础上,采用正交实验优化了工艺条件,对提取产物多糖进行了DPPH·、ABTS+·清除研究。结果表明:对多糖提取率影响最大的是提取次数,其次是液料比,提取时间影响最小;最佳提取工艺条件为提取3次,液料比20∶1mL·g~(-1),超声功率300 W,超声温度70℃,超声时间40 min,在此条件下多糖提取率达6.27%。多糖对DPPH·、ABTS+·有一定的清除作用,说明红蜡蘑多糖具有一定的抗氧化活性。
译 名：
Ultrasonic-assisted Extraction and Antioxidant Activity of Polysaccharides From Laccaria laccata
作 者：
ZHANG Shuihua;LI Benhong;ZHOU Weidong;Faculty of Chemistry and Environmental Science,Qujing Normal College;Library,Qujing Normal College;
关键词：
Laccaria laccata;;polysaccharides;;ultrasonic;;DPPH·;;ABTS+·
摘 要：
Taking Laccaria laccata as the raw material,the effects of extraction conditions such as ratio of liquid-solid,ultrasonic time,ultrasonic power,ultrasonic temperature and time of extraction were investigated by one-factor tests and orthogonal test,and the extraction polysaccharides were analyzed with DPPH· and ABTS+· test.The results showed that the times of extraction affected the yield markably,the second was ratio of liquid-solid,and ultrasonic time had the least influence;the optimum coditions were determined as follows:times of extraction of 3 times,ratio of liquid-solid of20∶1 mL·g~(-1),ultrasonic power of 300 W,ultrasonic temperature of 70 ℃ and ultrasonic time of 40 minutes.Under the condition,the yield was up to 6.27%.Polysaccharides exhibited antioxidant effects in 2,2′-diphenyl-1-picrylhydrazyl(DPPH·),2,2′-azino-bis(3-ethylben-othiazoline-6-sulfonic acid(ABTS+·)radical scavenging assay.And it showed that the extraction polysaccharides which had some oxidation resistance.
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