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Position: Home > Articles > Heterosis of restorer rice lines integrated with Cry1 Ab gene and its effect on insect resistance Chinese Journal of Eco-Agriculture 2007,15 (5) 147-150

Cry1 Ab转基因水稻的杂种优势表现及抗虫性鉴定

作  者:
戴正元;李爱宏;张洪熙;左示敏;舒庆尧
单  位:
江苏里下河地区农业科学研究所;扬州大学农学院;浙江大学核农业科学研究所
关键词:
水稻;Cry1Ab基因;恢复系;杂种优势;抗虫供体;抗虫性鉴定
摘  要:
以携带Cry1Ab基因的"克螟稻"为抗虫供体亲本,将目标基因转导到优良恢复系"R6547"、"R818"中,配制的杂交稻组合表现良好的杂种优势水平,克服了"克螟稻"在常规粳稻育种实践中常出现的农艺性状差、前期生长势弱的缺陷。可溶性蛋白含量检测结果显示目标基因在杂交稻中仍能高水平的表达,并在人工接虫和自然发生条件下对稻纵卷叶螟、二化螟等鳞翅目害虫表现优良抗性。
译  名:
Heterosis of restorer rice lines integrated with Cry1 Ab gene and its effect on insect resistance
作  者:
DAI Zheng-Yuan1,LI Ai-Hong1,ZHANG HongXi1,ZUO Shi-Min2,SHU Qing-Yao3(1.Lixiahe Institute of Agricultural Science,Jiangsu Province,Yangzhou 225007,China;2.College of Agriculture,Yangzhou University,Yangzhou 225007,China;3.Institute of Nuclear Agricultural Science,Zhejiang University,Hangzhou 310029,China),
关键词:
Rice,Cry1Ab gene,Restorer line,Heterosis,Insect-resistant donor,Evaluation of insect-resistance
摘  要:
Selecting Cry1Ab genecarrying rice germplasm "Kemingdao" as the insect-resistant donor,we transferred the target gene into two elite rice restorer lines"R6547"and "R818" through crossing and backcrossing.The hybrid combinations derived from two transgenic restorer lines show significant heterosis that do overcome the defects of undesirable agronomic traits and weak prophase development capabilities.Results of testing the Cry1Ab protein indicate that the target gene expresses strongly and provides good resistance to stripe stem borers(Chilo suppressalis) and leaf folders(Cnapha locrocis medinalis) in hybrid combinations.

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