Lin XueRong;Xia Yin;Yanyan Wang;Ji Minghu

College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou,Hainan 571158,China;College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou,Hainan 571158,Chin

paederia scandens;stem;microwave;ms;assisted;g

[Objective] To study the microwave-assisted cyclohexane optimum extraction conditions of volatile oil from the stem of Paederia scandens and analyze their contents by GC-MS,so as to provide references for the scientific utilization of Paederia scandens.[Method] The volatile oil was extracted from the stem of Paederia scandens by microwave-assisted cyclohexane extraction,then analyzed by GC-MS,the optimum conditions were screened by orthogonal experiment.[Result] The optimum extraction conditions were as follows: MPaederia scandens stem∶ Vcyclohexane=2∶ 60(g/ml),microwave extraction for 10 min utes,microwave power 800 W,and microwave temperature 50 ℃,under which the volatile oil yield reached 1.32%.10 compositions were detected from the stem extracts of Paederia scandens by GC-MS,of which seven compositions were compounds,accounting for 90.690% of the total peak area,the main components in the extracts were n-palmitic acid(18.424%),Phthalic acid isobutyl nonyl ester(24.243%) and oleic acid(15.131%).[Conclusion] The microwave-assisted cyclohexane extraction method of volatile oil form the stem of Paederia scandens was rapid with high oil yield,but the isolated components were too few,therefore the method needed to be further improved.