中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 樱桃谷鸭胸肉脂肪氧合酶的分离纯化及其酶学特性研究 食品科学 2013,34 (7) 166-170
Position: Home > Articles > Purification and Partial Characterization of Lipoxygenase in Cherry-valley Duck Breast Muscle FOOD SCIENCE 2013,34 (7) 166-170

樱桃谷鸭胸肉脂肪氧合酶的分离纯化及其酶学特性研究

作  者：
何立超;赵见营;田甜;章建浩
单  位：
国家肉品质量安全控制工程技术研究中心;华中农业大学楚天学院
关键词：
樱桃谷鸭;脂肪氧合酶;分离纯化;酶学特性
摘  要：
用硫酸铵分级沉淀和DEAE-Sepharose柱层析法分离纯化樱桃谷鸭胸肉中脂肪氧合酶(lipoxygenase,LOX),同时对酶学性质进行研究.结果显示:在DEAE-Sepharose柱层析时,当NaCl洗脱浓度在0.3~0.35mmol/L时,LOX被分离出,LOX经过分离纯化后其纯化倍数可达到54.3,SDS-PAGE电泳显示其分子质量约为96kD;粗酶和纯酶性质略有不同;以亚油酸为底物,粗酶和纯酶的最适底物浓度分别为6.4、10mmol/L,最适反应温度分别为40、30℃,最适pH值分别是5.0、5.5;纯酶Km=1.139mmol/L、Vmax=0.07608U/min,纯酶与底物的亲和能力大于粗酶.
译  名：
Purification and Partial Characterization of Lipoxygenase in Cherry-valley Duck Breast Muscle
单  位：
College of Food and Biotechnology,Chutian College,Huazhong Agricultural University,Wuhan 430205,China%National Center of Meat Quality and Safety Control,Key Laboratory of Meat Processing and Quality Control,Ministry of Education,Key Laboratory of Animal Products Processing and Quality Control,Ministry of Agriculture,College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China
关键词：
Cherry-valley duck%lipoxygenase(LOX)%isolation and purification%enzymatic characteristics
摘  要：
Crude lipoxygenase(LOX) from Cherry-valley duck breast muscle was purified through 20%-40% ammonium sulfate fractionation and DEAE-Sepharose column chromatography.LOX was separated by gradient elution of DEAESepharose column with 0.3-0.35 mmol/L NaCl solution,resulting in a purification factor of 54.3.The molecular weight of the purified LOX was measured by SDS-PAGE to be 96 kD.Slight differences in enzymatic properties between the crude LOX and the purified LOX were observed.Using linoleic acid as the substrate,the optimal substrate concentrations,reaction temperatures and pH values were 6.4 mmol/L and 10 mmol/L,40 ℃ and 30 ℃,and 5.0 and 5.5,respectively,and the Michaelis constant(Km) and maximum velocity(Vmax) for the purified LOX were 1.139 mmol/L and 0.07608 U/min,respectively.The purified LOX had a higher affinity for the substrate than the crude LOX.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46