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Position: Home > Articles > Determination of Total Sugar in Wujiaqi Powder Journal of Anhui Agricultural Sciences 2018,46 (33) 147-148+160

五加芪粉中总糖的含量测定

作  者:
张聪;樊丽博;杨会鲜;张继颖;姚路路;周冰;张晓会
单  位:
国家兽用药品工程技术研究中心洛阳惠中兽药有限公司
关键词:
五加芪粉;总糖含量;苯酚-硫酸比色法
摘  要:
[目的]建立五加芪粉中总糖的含量测定方法,以控制五加芪粉的质量。[方法]采用苯酚-硫酸比色法,检测波长为494 nm,以无水葡萄糖为对照品对五加芪粉中总糖的含量进行测定。[结果]无水葡萄糖对照品溶液的线性范围为0.342~1.035 mg/m L(r=0.999 4),平均加样回收率为98.5%,RSD=3.3%(n=6)。[结论]该研究确定的方法简单、可靠、准确,可用于测定五加芪粉中总糖的含量。
译  名:
Determination of Total Sugar in Wujiaqi Powder
作  者:
ZHANG Cong;FAN Li-bo;YANG Hui-xian;National Research Center for Veterinary Medicine,Luoyang Huizhong Veterinary Medicine Co.Ltd;
关键词:
Wujiaqi powder;;Total sugar content;;Phenol-sulfuric acid colorimetric method
摘  要:
[Objective]The research aimed to establish a method for determining the total sugar content in Wujiaqi powder to control the quality of Wujiaqi powder.[Method]The phenol-sulfuric acid colorimetric method was used,the detection wavelength was 494 nm,and the content of total sugar in Wujiaojiao powder was determined by using anhydrous glucose as the reference substance.[Result]The linear range of the anhydrous glucose reference solution was 0.342-1.035 mg/m L(r = 0.9994),the average recovery was 98.5%,and the RSD was 3.3%(n = 6).[Conclusion]The method identified in this study is simple,reliable and accurate,and can be used to determine the total sugar content of Wujiaqi powder.

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