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Position: Home > Articles > Cell Culture In Vitro Ylang-ylang(Cananga odorata)and Composition Analysis of the Oil from its Calli Chinese Journal of Tropical Crops 1998 (1) 34-41

依兰香细胞离体培养及其精油成分分析

作  者:
张树珍;曾宪松;张银东;郑学勤;陈惠吟
单  位:
中国热带农业科学院热带作物生物技术国家重点实验室
关键词:
依兰香;细胞培养;单细胞克隆;精油成分分析
摘  要:
以依兰香(Canangaodorata)的花瓣及雄蕊群为外植体,在1/2MS+2,4-D1-2+Kt0.2的培养基上分别诱导出白色和红色的两种愈伤组织,建立了依兰香细胞的大量培养(包括固体培养,悬浮培养及发酵罐培养)体系和单细胞克隆,通过化学方法进行高产油细胞系的初步筛选,从培养细胞中分离出依兰油,对油成分作了详细的分析。
译  名:
Cell Culture In Vitro Ylang-ylang(Cananga odorata)and Composition Analysis of the Oil from its Calli
作  者:
Zhang Shuzhen; Zeng Xiansong; Zhang Yindong; Zheng Xueqin; Chen Huiying(National Key Biotechnology Lab for Tropical Crops,CATAS,Danzhou,Hainan 571737)
关键词:
Cananga odorata;cell culture;single cell clone
摘  要:
Petals and stamens of ylang-ylang (Cananga odorata)was used as explants andcultured on half strength MS medium supplemented with 1 -2 μ/L 2,4 -D and 0. 2μ/Lkt.White and red calli were induced respectively from these two kinds of explants. Asystem of massive culture of ylang-ylang cells was established,including solid culture,suspension cu1ture and fermenter culture,and sing1e cell clones were derived,High oilyielding clones were preliminarily screened by using chemical approaches. Ylang-ylangoil was isolataed from the cultured cells and its composition was fully analyzed.

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