译 名：
Expression of Bt Protein in Transgenic Insect-resistant Rice
作 者：
YU Zhi-jing et al ( Biotechnology Research Center of Jilin Academy of Agricultural Sciences,Changchun,Jilin 130033)
关键词：
Enzyme-linked immunosorbent assay ( ELISA) ; Transgenic pest-resistant rice; Bt protein
摘 要：
[Objective]The aim was to study the expression of Bt protein in transgenic pest-resistant rice. [Method] Enzyme-linked immunosorbent assay ( ELISA) was used to measure Bt protein expression in different parts of transgenic pest-resistant rice at same growth stage. [Result]Absolute content of Bt protein expression from high to low was as follows: leaves > immature seeds and glumes > roots > stems in different tissues of transgenic rice in grain-filling stage; Bt protein contents of transgenic rice changed a little in different growth stages ( tillering stage,booting stage,and grain-filling stage) ; in general,concentration of Bt protein declined a little in later growth stage,for which,the resistibility would not be influenced too much. [Conclusion] The experiment is of great importance for pest prevention and transgenic rice breeding.
