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当前位置: 首页 > 文章 > 用3种分型方法研究鸭疫里默氏菌的血清型 畜牧兽医学报 2005,36 (3) 258-263
Position: Home > Articles > Studies on Serotype of Riemerella anatipestifer by Using Three Serotyping Methods Chinese Journal of Animal and Veterinary Sciences 2005,36 (3) 258-263

用3种分型方法研究鸭疫里默氏菌的血清型

作  者：
张大丙
单  位：
中国农业大学动物医学院
关键词：
鸭疫里默氏菌;血清型;分型方法;交叉反应;单因子血清
摘  要：
用玻片凝集试验、试管凝集试验和琼脂扩散沉淀试验 3 种方法,检测了鸭疫里默氏菌的 19 个参考菌株和部分分离株与同型和异型抗血清的反应。结果表明,3种分型方法具有很好的相关性,但在检测异型菌株之间的交叉反应时表现出不同。玻片凝集试验适于对大量分离株的快速筛选,但不能作出准确定型。以各型参考菌株为参照,根据试管凝集试验可将被检菌株分型,但当分离株与一个以上血清型的抗血清产生差异较小的凝集效价时,则难以定型。琼扩试验也适合于对分离株进行进一步检测,但要求制备较高效价的抗血清。对血清进行吸收可消除3种分型试验中的所有交叉反应,并制备出单因子血清。但这些单因子血清只是相对于已知血清型具有特异性,某些分离株虽然只与某型单因子血清发生凝集反应,仍可能属于新的血清型或某个已知型的亚型。
译  名：
Studies on Serotype of Riemerella anatipestifer by Using Three Serotyping Methods
作  者：
ZHANG Da-bing (College of Veterinary Medicine, China Agricultural University, Beijing 100094, China)
关键词：
Riemerella anatipestifer; serotype;serotyping method;cross reaction;mono-specific antiserum
摘  要：
representative type strains and some isolates of Riemerella anatipestifer were detected by slide and tube agglutination and agar gel precipitin tests. Homologous and heterologous reactions of R. anatipestifer strains showed that three serotyping methods correlated well, but gave differences on detecting cross reactions between heterologous stains.Slide agglutination test is a convenient method to screen large number of isolates quickly, but it is not suited to correctly serotype isolates. Isolates could be serotyped by tube agglutination test using type strains as control, but it is difficult to serotyped isolates which produced titers with minor dilution difference with antisera to more than one representative strains. Agar gel precipitin test is also suitable for further detection of isolates, but antiserum with higher-level antibodies should be prepared. Serum Absorption removed all cross-reactions in three serotyping tests, and mono-specific antiserum could be prepared. However, all mono-specific antiserum showed serotype-specificity only against the known serotypes. Further investigations indicated that some isolates which agglutinated in mono-specific antiserum against one serotype could belong to a new serotype or sub-serotypes of a known serotype.

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