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当前位置: 首页 > 文章 > 大豆GmAP1基因的功能初探 生物技术通报 2016,32 (8) 84-89
Position: Home > Articles > Functional Analysis of Soybean GmAP1 Biotechnology Bulletin 2016,32 (8) 84-89

大豆GmAP1基因的功能初探

作  者：
宋拉拉;张晓玫;陈福禄;傅永福;赵德刚
单  位：
中国农业科学院作物科学研究所;贵州大学农业生物工程研究院
关键词：
开花;花萼;GmAP1;MADS基因;大豆
摘  要：
以大豆天隆1号为材料,根据Glycine max Wm82.a2.v1中大豆基因组的预测序列,克隆出一个AP1同源基因,命名为GmAP1。该基因编码区CDS长度为711 bp,编码一个含236个氨基酸的蛋白质。对蛋白质序列的结构分析结果显示GmAP1蛋白符合MADS-box基因家族特征。为了初步分析GmAP1的功能,利用实时定量RT-PCR分析GmAP1在不同花器官中表达,并且在拟南芥中过表达该基因。在不同花器官中,GmAP1表达量不同,在花萼中表达量较高;花瓣次之。GmAP1过表达植株表现出早花、植株矮小以及花器官(花瓣、雄蕊和雌蕊)数量增多等表型。结果表明,大豆的GmAP1是一个功能保守的AP1基因,在花的发生和花器官发育中起着重要作用。
译  名：
Functional Analysis of Soybean GmAP1
作  者：
SONG La-la;ZHANG Xiao-mei;CHEN Fu-lu;FU Yong-Fu;ZHAO De-gang;The Key Lab of Plant Resources Conservation and Germplasm Innovation in Mountainous Region,Ministry of Education,Institute of Agrobioengineering and College of Life Sciences,Guizhou University;MOA Key Lab of Soybean Biology(Beijing),National Key Facility of Crop Gene Resource and Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences;
关键词：
flowering;;sepals;;GmAP1;;MADS gene;;soybean
摘  要：
The full length GmAPl gene,a homologous gene of API,was cloned from the soybean cultivar Tianlong 1 based on the putative sequence of soybean genome in Glycine max Wm82.a2.vl database.GmAPl was 711 bp in length and encoded a protein of 236 amino acids.The structure analysis of protein sequence showed that GmAPl protein characterized as MADs-box gene family.To analyze the function of GmAP1,the expression patterns of GmAP1 in different floral organs were analyzed by RT-PCR,and GmAP1 was overexpressed in Arabidopsis.The results showed that GmAP1 differently expressed in different floral organs with the highest level in sepals,following by the petals.Transgenic Arabidopsis plants displayed phenotypes of early flowering,dwarf,and increasing number of sepals,petals,and stamens.The result indicated that GmAPl was one of API homologous genes in soybean with conserved function and played an important role in flowering and floral organ development morphogenesis.

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