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当前位置: 首页 > 文章 > 达里诺尔湖和岗更湖瓦氏雅罗鱼同工酶的研究 水生态学杂志 2010,03 (6) 82-89
Position: Home > Articles > Study of Isoenzymes of Leuciscus waleckii in Dalinor & Ganggeng Lake Journal of Hydroecology 2010,03 (6) 82-89

达里诺尔湖和岗更湖瓦氏雅罗鱼同工酶的研究

作  者：
张建明;张玉;刘海涛
单  位：
内蒙古农业大学动物科学学院;内蒙古大学生命科学学院;内蒙古自治区水产技术推广站
关键词：
瓦氏雅罗鱼;同工酶;达里诺尔湖;岗更湖
摘  要：
采用聚丙烯酰胺凝胶电泳法(PAGE)比较研究了岗更湖(淡水)和达里诺尔湖(盐碱水)中瓦氏雅罗鱼(Leu-ciscus waleckii)的同工酶,旨在明确该品种在盐碱水域和淡水水域中同工酶的不同,同时也为瓦氏雅罗鱼在内陆水域的引种与放流提供科学依据。试验研究了两湖中瓦氏雅罗鱼骨骼肌、肝脏、心肌组织中过氧化物酶(POD)、酯酶(EST)、乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH)。结果表明,两湖中瓦氏雅罗鱼的过氧化物酶(POD)、酯酶(EST)、乳酸脱氢酶(LDH)同工酶存在差异性,而苹果酸脱氢酶(MDH)的差异不显著。
译  名：
Study of Isoenzymes of Leuciscus waleckii in Dalinor & Ganggeng Lake
作  者：
ZHANG Jian-ming1,ZHANG Yu1,2,LIU Hai-tao3 ( 1. Inner Mongolia Agricultural University Animal Science Institute,Hohhot 010018,China; 2. Inner Mongolia University,Life Sciences Institute,Hohhot 010021,China; 3. Inner Mongolia Aquaculture Technology Promotion Stations,Hohhot 010010,China)
关键词：
Leuciscus waleckii; Isoenzymes; Dalinor Lake; Ganggeng Lake
摘  要：
Leuciscus waleckii is a fish that can undertake salinity strongly,in this paper comparatively studied Isoenzymes of Leuciscus waleckii live in salt water and fresh waters with polyacrylamidegel electrophoresis ( PAGE) , to find difference of Isoenzymes of Leuciscus waleckii live in Dalinor Lake ( salt water) and Ganggeng Lake ( fresh water) . By studying,to provide basic research for mechanism of salinity and give scientific guidance for introduced in inland waters and release of Leuciscus waleckii. Through a comparative study peroxidase ( POD) ,esterase ( EST) ,lactate dehydrogenase ( LDH) and malic acid de-hydrogenase ( MDH) in muscle,liver and heart tissue of Leuciscus waleckii,the results obtained: Leuciscus waleckii with differences in expression of the isozymes in the two lakes,and EST mapping has the biggest differences between them,LDH and POD are also have differences in the pattern,but MDH pattern no significant difference. Experiments show that peroxidase ( POD) ,esterase ( EST) and lactate dehydrogenase ( LDH) of Leuciscus waleckii have differences in two lakes,whereas the difference of malic acid de-hydrogenase ( MDH) was not significant.

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