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当前位置: 首页 > 文章 > 外源激素对杂交兰组培快繁的效应 南方农业学报 2013,44 (9) 1435-1438
Position: Home > Articles > Effects of exogenous growth regulators on rapid propagation of Cymbidium hybridum × faberi Journal of Southern Agriculture 2013,44 (9) 1435-1438

外源激素对杂交兰组培快繁的效应

作  者：
秦丽凤;石贵玉
单  位：
广西师范大学生命科学学院;桂林市林业科学研究所
关键词：
杂交兰;组织培养;原球茎;激素;效应
摘  要：
【目的】探讨杂交兰(Cymbidium hybridum×faberi)组培快繁技术,为杂交兰的工厂化生产提供技术支撑。【方法】以杂交兰茎尖和腋芽为外植体,调查不同激素与活性炭(AC)组合对原球茎诱导、增殖分化和生根培养的影响。【结果】6-BA是影响原球茎诱导的主要因素,IBA是影响原球茎增殖和分化的主要因素。原球茎诱导和增殖最适培养基为MS+6-BA 1.0 mg/L+IBA 0.5 mg/L+AC 3.0 g/L+3%蔗糖;MS+6-BA 0.5 mg/L+IBA 0.5 mg/L+AC 3.0 g/L+3%蔗糖最适于原球茎分化成苗;壮苗生根培养基为1/2MS+IBA 0.5 mg/L+AC 1.0 g/L+3%蔗糖+0.5%琼脂。【结论】在MS基本培养基中,低量的6-BA、IBA对杂交兰原球茎诱导、增殖与分化、生根和移栽成活均有明显促进效应,原球茎不同生长阶段对AC的需求量不同。
译  名：
Effects of exogenous growth regulators on rapid propagation of Cymbidium hybridum × faberi
作  者：
QIN Li-feng;SHI Gui-yu;Guilin Research Institute of Forestry Sciences;College of Life Sciences,Guangxi Normal University;
关键词：
Cymbidium hybridum × faberi;;tissue culture;;protocorm;;hormone;;effect
摘  要：
【Objective】Cymbidium hybridum × faberi rapid propagation technology was explored to provide technical support for large-scale Cymbidium hybridum × faberi seedling production. 【Method】Using stem tip and axillary bud of Cymbidium hybridum × faberi as the explants, the effects of different growth regulators and active carbon(AC) on protocorm induction, proliferation and differentiation, and rooting culture were studied. 【Result】The main factor concerning protocorm induction was 6-BA, and IBA was the dominant element influencing protocorm proliferation and differentiation. The optimal medium for induction and propagation of the protocorm was MS+6-BA 1.0 mg/L+IBA 0.5 mg/L+AC 3.0 g/L+3% sugar. MS+6-BA 0.5 mg/L+IBA 0.5 mg/L+AC 3.0 g/L+3% sugar was propitious to differentiation of protocorm. The optimal medium on rooting was 1/2MS+IBA 0.5 mg/L+AC 1.0 g/L+3% sugar+0.5% agar. 【Conclusion】It was concluded that MS culture medium adding 6-BA and IBA increased protocorm induction rate, protocorm multiplication times, protocorm differentiation rate,rooting rate and transplant survival rate of Cymbidium hybridum × faberi seedlings. The demand for AC amount varied at different growth stages of protocom.

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