Position: Home > Articles > Development of colloidal gold immunochromatographic assays for rapid detection of Escherichia coli O_(157) and Stx2
Chinese Veterinary Science
2009,39
(2)
154-158
大肠杆菌O_(157)及其Stx2免疫胶体金层析检测方法的建立
作 者：
高成秀;孙建和;严亚贤
单 位：
上海交通大学农业与生物学院上海市兽医生物技术重点实验室
关键词：
免疫胶体金层析;大肠杆菌O157;志贺毒素2
摘 要：
建立了大肠杆菌O157和志贺毒素2(Stx2)的双抗体夹心免疫胶体金层析法,并运用该方法对上海市兽医生物技术重点实验室保存的已鉴定的58株大肠杆菌进行了检测,评估了其特异性、敏感性和重复性。结果显示,大肠杆菌O157层析法的敏感性为1×105CFU/mL,特异性为87.5%,批间和批内变异系数分别为8.6%和0;Stx2层析法的检测下限为200μL细菌培养液,特异性为50.0%,批间和批内变异系数分别为50.0%和33.0%。表明,该免疫胶体金层析法能快速、简便、特异地检测大肠杆菌O157;结合对Stx2的检测,能快速检出产Stx2的大肠杆菌O157菌株。
译 名：
Development of colloidal gold immunochromatographic assays for rapid detection of Escherichia coli O_(157) and Stx2
作 者：
GAO Cheng-xiu,SUN Jian-he,YAN Ya-xian(1.Shanghai Key Laboratory of Veterinary Biotechnology/School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 201101,China)
关键词：
colloidal gold immunochromatography;Escherichia coli O157;Shiga toxin 2(Stx2)
摘 要：
Two colloidal gold immunochromatographic tests were developed based on double-antibody sandwich assay for rapid detecting Escherichia coli(E.coli) O157 and Shiga toxin 2(Stx2),respectively.The sensitivities,specificities and correlations were evaluated with 58 known E.coli strains in Shanghai Key Laboratory of Veterinary Biotechnology.The sensitivity and specificity of the test for E.coli O157 were 1×105 CFU/mL,and 87.5%,respectively.Coefficient of variation(CV) of the test for E.coli O157 were 8.6% and 0 in inter-assay and intra-assay,respectively.The detection limit of the test for Stx2 was 200 μL bacterial culture.The specificity of the test for Stx2 was 50.0%.CV of the test for Stx2 were 55.0% and 33.0% in inter-assay and intra-assay,respectively.The results revealed that the colloidal gold immunochromatographic assay for detection of E.coli O157 was rapid,convenient and specific,and could rapidly detect the Stx2-producing E.coli O157 in combination with the colloidal gold immunochromatographic test for detection of Stx2.
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