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Position: Home > Articles > PRKAG3 Gene Expression Difference in Tissues and Organs and the Relationships between Its Relative Expression and Carcass Traits in Pigs Chinese Journal of Animal and Veterinary Sciences 2006,37 (6) 566-570

PRKAG3在猪组织器官中的表达差异及与胴体品质关系研究

作者：

李梦云;陈代文;张克英

单位：

四川农业大学动物营养研究所

关键词：

PRKAG3;猪;组织器官;基因表达;胴体品质

摘要：

PRKAG3是编码一磷酸腺苷激活蛋白激酶(AMP-activated protein kinase,AMPK)γ3亚基的基因,是近年来确定的一个影响猪肉pH值、肉色以及系水力的主效基因,其突变R200Q与过量肌糖原含量有关,被认为是汉普夏猪发生RN-突变的根本原因。本研究以-βactin作为内参,用半定量RT-PCR方法测定雅南猪和杜×长×大三元杂交猪(DLY猪)PRKAG3基因在心脏、肝脏、肾和骨骼肌中表达差异,并测定猪胴体品质。结果表明,PRKAG3主要在骨骼肌中表达,在心脏中有少量表达,而在肝脏和肾中未见表达,在不同品种的骨骼肌中,雅南猪PRKAG3基因的相对表达量高于DLY猪(P=0.078)。相关分析结果表明:骨骼肌中PRKAG3基因的表达量与屠宰率、眼肌面积和背膘厚关系不大,而与肌内脂肪含量成正相关(r=0.832)。

译名：

PRKAG3 Gene Expression Difference in Tissues and Organs and the Relationships between Its Relative Expression and Carcass Traits in Pigs

作者：

LI Meng-yun,CHEN Dai-wen~*,ZHANG Ke-ying (Institute of Animal Nutrition,Sichuan Agricultural University,Yaan 625014,China)

关键词：

PRKAG3;pigs;tissues and organs;gene expression;carcass traits

摘要：

PRKAG3 gene,which encodes AMP-activated protein kinase(AMPK)γ3 subunit,was considered as a major gene of affecting meat quality traits such as muscle pH,color and water-holding capacity,and its nonconservative substitution(R200Q) associated with excess glycogen content in pig skeletal muscle is the reason of causing RN~-effect.In present study,semi-quantitative RT-PCR was applied to determine the PRKAG3 gene expression difference in heart,liver,kidney and skeletal muscle of Ya-Nan and DLY pigs,and the data were normalized using the βactin gene as internal control.Additionally,carcass traits,including undressing percentage,backfat thickness,eye lean area and intramuscular fat were measured.The results suggested that PRKAG3 was mainly expressed in skeletal muscle and less in heart,but not in liver and kidney.In skeletal muscle,PRKAG3 gene expression of Ya-Nan pigs was higher than that of DLY pigs(P=0.078).Correlation analyses showed that the relative expression of PRKAG3 gene had no relation to undressing percentage,backfat thickness and eye lean area,but had positive relation to intramuscular fat(r=0.832).

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PRKAG3在猪组织器官中的表达差异及与胴体品质关系研究

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