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当前位置: 首页 > 文章 > 高效液相色谱手性拆分法分析生物体内虾青素光学异构体 食品科学 2015,36 (8) 139-144
Position: Home > Articles > Chiral Separation and Analysis of Astaxanthin Stereoisomers in Biological Organisms by High-Performance Liquid Chromatography FOOD SCIENCE 2015,36 (8) 139-144

高效液相色谱手性拆分法分析生物体内虾青素光学异构体

作  者：
杨澍;张婷;徐杰;李学敏;周庆新;薛长湖
单  位：
中国海洋大学食品科学与工程学院
关键词：
虾青素;光学异构体;手性分析;高效液相色谱
摘  要：
建立一种高效液相色谱手性拆分法分析不同生物中3种虾青素光学异构体含量的方法。以Chiralpak IC色谱柱(4.6 mm×250 mm,5 μm)为固定相,以甲基叔丁基醚-乙腈(95∶5,V/V)为流动相,采用等度洗脱,流速1.0 m L/min,二极管阵列检测器检测波长为476 nm,选用雨生红球藻、红法夫酵母、南美白对虾、中国对虾、日本对虾、三文鱼、梭子蟹7种生物样品为研究对象,分析其虾青素光学异构体,线性范围为0.1~50 mg/L,相关系数为0.999 9,回收率为86.2%~98.3%,方法稳定性良好。结果表明,该方法可行、快速简便、准确可靠。
译  名：
Chiral Separation and Analysis of Astaxanthin Stereoisomers in Biological Organisms by High-Performance Liquid Chromatography
作  者：
YANG Shu;ZHANG Ting;XU Jie;LI Xuemin;ZHOU Qingxin;XUE Changhu;College of Food Science and Engineering, Ocean University of China;
关键词：
astaxanthin;;stereoisomers;;chiral analysis;;high-performance liquid chromatography (HPLC)
摘  要：
A high performance liquid chromatography (HPLC) method for chiral separation and analysis of astaxanthin stereoisomers in several biological organisms was developed. The separation was performed on a Chiralpak IC column (4.6 mm × 250 mm, 5 μm) by using methyl tert-butyl ether (MTBE)-acetonitrile as the mobile phase at a flow rate of 1.0 m L/min. The detection wavelength of diode array detector (DAD) was 476 nm. This method was applied to analyze astaxanthin stereoisomers in Haematococcus pluvialis, Phaffia yeast, Penaeus vannamei, Penacus orientalis, Japanese tiger prawn, salmon and crab. The results showed that the linear range of the calibration curve for astaxanthin stereoisomers was 0.1–50 mg/L with a correlation coefficient of 0.999 9. The average recovery rates for astaxantin stereoisomers were in the range of 86.2%–98.3%. These results demonstrate that the proposed method is feasible, rapid, simple and accurate.

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