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当前位置: 首页 > 文章 > 鸡排泄物中阿莫西林和氨苄西林残留同时检测的高效液相色谱荧光法 扬州大学学报(农业与生命科学版) 2015,36 (3) 36-40
Position: Home > Articles > Simultaneous determination of residues of amoxicillin and ampicillin in chicken excrement by HPLC with fluorescence detection Journal of Yangzhou University(Agricultural and Life Science Edition) 2015,36 (3) 36-40

鸡排泄物中阿莫西林和氨苄西林残留同时检测的高效液相色谱荧光法

作  者：
刘建宇;高强;谢恺舟;崔璐璐;刘亚楠;王波;张扬扬;张跟喜;戴国俊;王金玉
单  位：
扬州大学动物科学与技术学院/江苏省动物遗传繁育与分子设计重点实验室
关键词：
鸡;排泄物;阿莫西林;氨苄西林;残留;高效液相色谱;荧光检测法
摘  要：
为建立鸡排泄物中阿莫西林(AMO)和氨苄西林(AMP)残留同时检测的高效液相色谱荧光法,将鸡排泄物先经乙腈提取、饱和二氯甲烷萃取、酸性条件下经水杨醛沸水浴衍生化后,以0.01mol·L-1磷酸二氢钾溶液(A)和乙腈溶液(B)为流动相,梯度洗脱,流速为1.0mL·min-1,荧光检测激发波长为354nm、发射波长为445nm。当AMO和AMP浓度为0.1~250.0μg·g-1时,该方法呈良好的线性相关,相关系数均为0.999 8。当AMO和AMP添加水平为5.0~125.0μg·g-1时,鸡排泄物中AMO和AMP的回收率均高于71.52%,相对标准偏差均低于9.77%。AMO检测限(S/N=3)为3.0ng·g-1、定量限(S/N=10)为6.0ng·g-1;AMP检测限(S/N=3)为1.2ng·g-1、定量限(S/N=10)为3.5ng·g-1。该方法简便、灵敏、准确、快速,可满足鸡排泄物中AMO和AMP残留同时提取和检测的要求。
译  名：
Simultaneous determination of residues of amoxicillin and ampicillin in chicken excrement by HPLC with fluorescence detection
作  者：
LIU Jianyu;GAO Qiang;XIE Kaizhou;CUI Lulu;LIU Yanan;WANG Bo;ZHANG Yangyang;ZHANG Genxi;DAI Guojun;WANG Jingyu;Coll of Anim Sci and Tech/Key Lab for Anim Gen,Breeding,Reprod and Molr Design of Jiangsu Prov,Yangzhou Univ;
关键词：
chicken;;extrement;;amoxicillin;;ampicillin;;residues;;high efficiency liquid chromatography;;fluorescence detection
摘  要：
A high performance liquid chromatography with fluorescence detection method(HPLC-FLD)was developed for simultaneous determination of residues of amoxicillin(AMO)and ampicillin(AMP)in chicken excrement.AMO and AMP in chicken excrement were extracted with acetonitrile and saturated dichloromethane,and the derivatization was in salicylaldehyde/water bath(acid environment).The mobile phase was phosphate potassium dihydrogen solution(A)and acetonitrile(B).The gradient elution was at a flow rate of 1.0mL·min-1.The fluorescence was performed at an excitation wavelength of 354 nm and an emission wavelength of 445 nm.The concentrations of AMO and AMP were proved with good linear correlation at the range of 0.1to 250.0μg·g-1 and the correlation coefficients were 0.999 8for AMO and 0.999 8for AMP.At spiked levels of 5.0to 125.0μg·g-1 for AMO and AMP,sample recoveries ranged were higher than 71.52%and RSD were less than 9.77%.The detection and quantification for AMO was 3.0ng·g-1(S/N=3)and 6.0ng·g-1(S/N=10),respectively.The limits of detection and quantification for AMP was 1.2ng·g-1(S/N=3)and 3.5ng·g-1(S/N=10),respectively.This method is sensitive,accurate,simple,and rapid,and can meet the simultaneous detection of AMO and AMP residue in chicken excrement.

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