单 位：
扬州大学医学院;江苏大学医学技术学院;北华大学医学院
关键词：
SGC-7901细胞;亚硒酸钠;分裂指数;细胞周期;细胞凋亡
摘 要：
目的:研究亚硒酸钠对SGC-7901细胞生长的影响。方法:应用细胞培养和SRB实验探讨了亚硒酸钠对SGC-7901细胞生长曲线的影响;应用集落形成试验、分裂指数试验研究了亚硒酸钠对SGC-7901细胞集落形成和分裂指数的影响;应用流式细胞仪检测了亚硒酸钠对SGC-7901细胞生长周期的影响;应用电镜、TUNEL染色及流式细胞仪观察了亚硒酸钠诱导SGC-7901细胞凋亡的作用。结果:亚硒酸钠溶液对SGC-7901细胞的生长曲线、集落形成、分裂指数有明显的抑制作用,其抑制作用与其作用浓度和作用时间呈正相关。流式细胞仪检测显示亚硒酸钠作用SGC-7901细胞24h后,细胞周期发生改变,G1期细胞百分率减少,S期细胞百分率增加,与对照组相比有显著性差异(p<0.05);电镜下,细胞核固缩,染色质凝集呈新月形紧贴于核膜周边,核膜扭曲;DNA直方图上出现典型的亚二倍体的“凋亡峰”;TUNEL染色法检测细胞凋亡指数在10.4%～33.4%。结论:亚硒酸钠溶液对SGC-7901细胞的生长具有抑制作用,其抑制作用程度与其作用浓度和时间呈正相关;亚硒酸钠通过阻滞细胞S期抑制细胞增殖及诱导细胞凋亡可能是其抑制SGC-7901细胞生长的机理之一。
译 名：
Inhibitory Effect of Sodium Selenite on the Growth of SGC-7901 Cells
作 者：
SHAO Shi-he1,SUN Li-yuan2,LI Guo-li3,DUAN Xiu-jie1 (1.School of Medicine, Jiangsu University, Zhenjiang 212001, China;2.School of Medicine, Beihua University, Jilin 132001, China;3.School of Medicine, Yangzhou University, Yangzhou 225001, China)
关键词：
SGC-7901 cell;sodium selenite;mitotic index;cell cycle;cell apoptosis
摘 要：
Objective: To examine the effect of sodium selenite on growth of SGC-7901 cells. Methods: The growth curve of SGC-7901 cells was measured by using cell culture and SRB experiment; effects of sodium selenite on SGC-7901 cell colony- forming and cell mitotic index were examined by using the relative tests; effect of sodium selenite on SGC-7901 cell growth cycle was measured by using flow cytometry; and sodium selenite-induced SGC-7901 cell apoptosis was examined by electron microscope, TUNEL staining and flow cytometry. Results: In the curve sodium selenite significantly inhibited respectively the SGC-7901 cell growth, colony-forming and mitotic index dose dependently. Flow cytometry detection showed that, after 24 hours, sodium selenite reduced the percentage of G1 phase cells but increased the percentage of S phase cells in cell cycle (p< 0.05 compared with control group). Electron microscope detection showed nucleus pycnosis, and crescent-shaped chromatic agglutination stiching closed to karyotheca with karyotheca distortion. DNA histogram showed a typical apoptosis peak as hyodiplod. TUNEL staining showed that apoptosis index was between 10.4% and 33.4%. Conclusion: Sodium selenite had an inhibitory effect on SGC-7901 cell growth with dose-effect relationship. The inhibition of S phase cell proliferation and induction of cell apoptosis might underlie the mechanism of the inhibitory effect of sodium selenite on SGC-7901 cell growth.
