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当前位置: 首页 > 文章 > 兴国红鲤MHCⅡ类α基因多态性、表达及其与鱼体抗病力关系的分析 水生生物学报 2014 (2) 241-248
Position: Home > Articles > THE ANALYSIS OF THE POLYMORPHISM AND EXPRESSION OF MHC CLASS IIα GENE AND ITS ASSOCIATION WITH RESISTANCE/SUSCEPTIBILITY TO AEROMONAS HYDROPHILA IN XINGGUO RED COMMON CARP Acta Hydrobiologica Sinica 2014 (2) 241-248

兴国红鲤MHCⅡ类α基因多态性、表达及其与鱼体抗病力关系的分析

作  者：
刘俊;刘至治;王成辉;项松平;王剑
单  位：
上海海洋大学农业部淡水水产种质资源重点实验室;上海海洋大学水产种质资源发掘与利用教育部重点实验室;浙江龙泉省级瓯江彩鲤良种场
关键词：
兴国红鲤;MHC;多态性;表达;抗病力
摘  要：
对41尾感病和22尾抗病兴国红鲤的308个有效克隆进行测序,获得171条不同的MHCⅡ类α基因编码序列,分属26个不同的等位基因,其中Cyca-DXA24—Cyca-DXA36为新发现的13个等位基因。MHCⅡ类α基因片段的长度为624 bp,包括第1—4个外显子,分别编码信号肽、α1和α2结构域及连接肽/跨膜区。α1结构域的变异明显大于α2结构域,表现在α1结构域中核苷酸和氨基酸变异位点比例(55.16%和79.76%)明显高于α2结构域的变异位点比例(45.96%和68.42%)。α1结构域的PBR区的非同义碱基替换率(dN)与同义碱基替换率(dS)的比值ω(ω=dN/dS)为5.742,远远高于非抗原结合位点(non-PBR)及α2结构域的0.755、0.592,揭示兴国红鲤MHCⅡ类α基因的α1结构域在进化过程中受到正向选择作用。等位基因Cyca-DXA24(P<0.01)与兴国红鲤对嗜水气单胞菌的抗性相关,等位基因Cyca-DXA3(P<0.05)、Cyca-DXA4(P<0.01)、Cyca-DXA6(P<0.05)、Cyca-DXA33(P<0.05)与兴国红鲤对嗜水气单胞菌的易感性相关。荧光定量PCR结果表明,MHCⅡ类α基因在健康兴国红鲤的肾、肝、鳃等10个组织均能普遍表达。人工感染嗜水气单胞菌后,肾、肝、脾3个组织中的MHCⅡ类α基因的表达量均发生了不同程度的变化,表明MHCⅡ类α分子在兴国红鲤的免疫反应中起到重要作用。
译  名：
THE ANALYSIS OF THE POLYMORPHISM AND EXPRESSION OF MHC CLASS IIα GENE AND ITS ASSOCIATION WITH RESISTANCE/SUSCEPTIBILITY TO AEROMONAS HYDROPHILA IN XINGGUO RED COMMON CARP
作  者：
LIU Jun;LIU Zhi-Zhi;WANG Cheng-Hui;XIANG Song-Ping;WANG Jian;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University,Ministry of Education;Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean UniversityMinistry of Agriculture;Oujiang Color Common Carp Provincial Farm;
关键词：
Cyprinus carpio var.singguoensis;;MHC;;Polymorphism;;Expression;;Disease resistance
摘  要：
We cloned 171 sequences of MHC class IIα gene were determined from 41 susceptible and 22 resistant individuals of Cyprinus carpio var. singguoensis. These sequences belonged to 26 alleles, thirteen of which were novel(Cyca-DXA24-Cyca-DXA36). The total length of the sequence was 624 bp, consisting of a 33 bp exon1, a 252 bp exon2, a 285 bp exon3, and a 54 bp exon4. These exons encoded a partial signal peptide, a α1 domain, a α2 domain and a connecting peptide(CP)/partial transmembrane(TM), respectively. The mutation-Prone positions of nucleotide or amino acid sites in α1 domain(55.16%, 79.76%) significantly out number than those in α2 domain(45.96%, 68.42%). The ω values(ω= dN/dS) were 5.742 for peptide-binding region(PBR), 0.755 for non-PBR in the α1 domain and 0.592 for the α2 domain, implying that the positive selection pressure probably had occurred in the PBR region of the α1 domain. Cyca-DXA24(P<0.01) was significantly correlated with the carp's resistance to Aeromonas hydrophila, while Cyca-DXA3(P<0.05), Cyca-DXA4(P<0.01), Cyca-DXA6(P<0.05) and Cyca-DXA33(P<0.05) alleles were significantly associated with the fish's susceptibility to the disease. Real-time quantitative PCR demonstrated that MHC IIα genes were ubiquitously expressed in ten tissues. The expression was up-regulated in kidney, down-regulated in liver and spleen during infection. Our results indicated that the MHC class IIα gene might play an important role in the immune reactions of C. carpio var. singguoensis.

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